NDLI: Combined image guided monitoring the pharmacokinetics of rapamycin loaded human serum albumin nanoparticles with a split luciferase reporter

Content Provider	Royal Society of Chemistry (RSC)
Author	Wang, Zhe Wang, Fu Yang, Kai Ma, Ying Niu, Gang Gutkind, J. Silvio Hida, Naoki Tian, Jie
Copyright Year	2016
Abstract	Imaging guided techniques have been increasingly employed to investigate the pharmacokinetics (PK) and biodistribution of nanoparticle based drug delivery systems. In most cases, however, the PK profiles of drugs could vary significantly from those of drug delivery carriers upon administration in the blood circulation, which complicates the interpretation of image findings. Herein we applied a genetically encoded luciferase reporter in conjunction with near infrared (NIR) fluorophores to investigate the respective PK profiles of a drug and its carrier in a biodegradable drug delivery system. In this system, a prototype hydrophobic agent, rapamycin (Rapa), was encapsulated into human serum albumin (HSA) to form HSA Rapa nanoparticles, which were then labeled with Cy5 fluorophore to facilitate the fluorescence imaging of HSA carrier. Meanwhile, we employed transgenetic HN12 cells that were modified with a split luciferase reporter, whose bioluminescence function is regulated by Rapa, to reflect the PK profile of the encapsulated agent. It was interesting to discover that there existed an obvious inconsistency of PK behaviors between HSA carrier and rapamycin in vitro and in vivo through near infrared fluorescence imaging (NIFRI) and bioluminescence imaging (BLI) after treatment with Cy5 labeled HSA Rapa. Nevertheless, HSA Rapa nanoparticles manifested favorable in vivo PK and tumor suppression efficacy in a follow-up therapeutic study. The developed strategy of combining a molecular reporter and a fluorophore in this study could be extended to other drug delivery systems to provide profound insights for non-invasive real-time evaluation of PK profiles of drug-loaded nanoparticles in pre-clinical studies.
Starting Page	3991
Ending Page	4000
Page Count	10
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	8
Issue Number	7
Journal	Nanoscale
DOI	10.1039/c5nr07308a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Biodegradation Human serum albumin Bioluminescence HSA Rapa Infrared Sirolimus Nanoparticle Rapa Luciferase Fluorescence Drug delivery Hydrophobe Reporter gene Fluorophore HSA Pharmacokinetics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Bovine serum albumin coated nanoparticles for in vitro activated fluorescence

Paclitaxel modified Fe₃O₄ loaded albumin nanoparticles as drug delivery vehicles by self-assembly

Characterisation and structure analysis of taxanes-loaded human serum albumin nanoparticles prepared by self-assembly method

Inhibition of fibrillation of human serum albumin through interaction with chitosan-based biocompatible silver nanoparticles

Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells.

Secured delivery of basic fibroblast growth factor using human serum albumin-based protein nanoparticles for enhanced wound healing and regeneration

Cabazitaxel-loaded human serum albumin nanoparticles as a therapeutic agent against prostate cancer

Cabazitaxel-loaded human serum albumin nanoparticles as a therapeutic agent against prostate cancer

Binding of a potential anti-hepatoma drug cis,cis,trans-[Pt(NH₃)₂Cl₂(O₂CCH₂CH₂COOH)-(OCONHC₁₆H₃₃)] with serum albumin – thermodynamic and conformational investigations

Combined image guided monitoring the pharmacokinetics of rapamycin loaded human serum albumin nanoparticles with a split luciferase reporter

Similar Documents

Bovine serum albumin coated nanoparticles for in vitro activated fluorescence

Paclitaxel modified Fe3O4 loaded albumin nanoparticles as drug delivery vehicles by self-assembly

Characterisation and structure analysis of taxanes-loaded human serum albumin nanoparticles prepared by self-assembly method

Inhibition of fibrillation of human serum albumin through interaction with chitosan-based biocompatible silver nanoparticles

Preparation, characterization and targeting of micronized 10-hydroxycamptothecin-loaded folate-conjugated human serum albumin nanoparticles to cancer cells.

Secured delivery of basic fibroblast growth factor using human serum albumin-based protein nanoparticles for enhanced wound healing and regeneration

Cabazitaxel-loaded human serum albumin nanoparticles as a therapeutic agent against prostate cancer

Cabazitaxel-loaded human serum albumin nanoparticles as a therapeutic agent against prostate cancer

Binding of a potential anti-hepatoma drug cis,cis,trans-[Pt(NH3)2Cl2(O2CCH2CH2COOH)-(OCONHC16H33)] with serum albumin – thermodynamic and conformational investigations

Combined image guided monitoring the pharmacokinetics of rapamycin loaded human serum albumin nanoparticles with a split luciferase reporter

Paclitaxel modified Fe₃O₄ loaded albumin nanoparticles as drug delivery vehicles by self-assembly

Binding of a potential anti-hepatoma drug cis,cis,trans-[Pt(NH₃)₂Cl₂(O₂CCH₂CH₂COOH)-(OCONHC₁₆H₃₃)] with serum albumin – thermodynamic and conformational investigations