NDLI: A self-assembly aptasensor based on thick-shell quantum dots for sensing of ochratoxin A

Content Provider	Royal Society of Chemistry (RSC)
Author	Kong, Weijun Zhao, Ming Luo, Jiaoyang Li, Menghua Chu, Xianfeng Yang, Meihua Yang, Xihui Dou, Xiaowen Liang, Ruizheng
Copyright Year	2016
Abstract	A novel self-assembling aptasensor was fabricated by precisely attaching three phosphorothioate-modified capture aptamers onto a single thick-shell quantum dot in a controllable manner for monitoring of ochratoxin A (OTA), a poisonous contaminant widespread in foodstuffs. Herein, CdSe/CdS QDs coated in ten layer CdS shells were synthesized using a continual precursor injection method. Analysis of the prepared CdSe/CdS QDs showed a zinc-blende structure, high photoluminescence quantum yields (>80%), and a photoemission peak with a narrow full-width at half-maximum (about 29 nm), all qualities that render them as a superior choice for optical applications. By adjusting the number of phosphorothioate bases in the anchor domain, the tunable-valency aptasensor was able to self-assemble. In the sensing strategy, the thick-shell quantum dot was provided as an acceptor while OTA itself was used as a donor. In the presence of OTA, the capture aptamers drive the aptasensor function into a measurable signal through a fluorescence resonance energy transfer (FRET) system. The newly developed aptasensor had a detection limit as low as 0.5 ng mL−1, with a linear concentration in the range of 1 to 30 ng mL−1, and therefore meets the requirements for rapid, effective, and anti-interference sensors for real-world applications. Moreover, the high quality thick-shell QDs provide an ideal alternative for highly sensitive imaging and intensive illumination in the fields of biotechnology and bioengineering.
Starting Page	4127
Ending Page	4133
Page Count	7
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	8
Issue Number	7
Journal	Nanoscale
DOI	10.1039/c5nr08284f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Photoelectric effect Förster resonance energy transfer Biotechnology Quantum dot Photoluminescence Fluorescence OTA Ochratoxin A Biological engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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