NDLI: In vivo investigation of the substrate recognition capability and activity affecting amino acid residues of glycosyltransferase FscMI in the biosynthesis of candicidin

Please wait, while we are loading the content...

Discovery and optimization of triazine derivatives as ROCK1 inhibitors: molecular docking, molecular dynamics simulations and free energy calculations

Pathway Pattern-based prediction of active drug components and gene targets from H1N1 influenza's treatment with maxingshigan-yinqiaosan formula

Inhibition mechanism exploration of quinoline derivatives as PDE10A inhibitors by in silico analysis

A system mathematical model of a cell–cell communication network in amyotrophic lateral sclerosis

Downregulated MEG3 activates autophagy and increases cell proliferation in bladder cancer

Structural characterization and biological properties of human gastrokine 1

In vivo investigation of the substrate recognition capability and activity affecting amino acid residues of glycosyltransferase FscMI in the biosynthesis of candicidin

¹H NMR-based metabolomics exploring biomarkers in rat cerebrospinal fluid after cerebral ischemia/reperfusion

The integrated response of primary metabolites to gene deletions and the environment

Redox-proteomic analysis of doxorubicin-induced altered thiol activity in cardiomyocytes

Studying the differential co-expression of microRNAs reveals significant role of white matter in early Alzheimer's progression

Network analysis of genomic alteration profiles reveals co-altered functional modules and driver genes for glioblastoma

Cloning and sequencing of the kedarcidin biosynthetic gene cluster from Streptoalloteichus sp. ATCC 53650 revealing new insights into biosynthesis of the enediyne family of antitumor antibiotics

Sequential selective enrichment of phosphopeptides and glycopeptides using amine-functionalized magnetic nanoparticles

Virus-like particles with removable cyclodextrins enable glutathione-triggered drug release in cells

Switching p53 states by calcium: dynamics and interaction of stress systems

Genome-wide transcriptional responses of Escherichia coli to glyphosate, a potent inhibitor of the shikimate pathway enzyme 5-enolpyruvylshikimate-3-phosphate synthase

Back cover

Molecular BioSystems Vol - 9 Issue - 2

Molecular BioSystems Vol -8

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

In vivo investigation of the substrate recognition capability and activity affecting amino acid residues of glycosyltransferase FscMI in the biosynthesis of candicidin

Content Provider	Royal Society of Chemistry (RSC)
Author	You, Delin Liu, Qian Zhang, Chen Lei, Xuan Deng, Zixin Zhang, Weike Kong, Lingxin Yao, Fen
Copyright Year	2013
Abstract	Alteration of sugar moieties of natural products often leads to novel antibiotics with different chemical and physical properties. fscMI is a putative glycosyltransferase (GT) in a gene cluster for the production of candicidin, a polyene macrolide antibiotic, produced by Streptomyces sp. FR-008. In this report, we established an in vivo biochemical detection system by inactivating fscMI and the DH11 domain of polyketide synthase (PKS) through double homologous recombination to unveil the interaction between polyene GTs and their substrates. We found that homologous GT genes including amphDI, nysDI and pimK can catalyze the conversion of candicidin aglycone into candicidin/FR-008-III in fscMI mutant, suggesting that homologous polyene GTs show some tolerance toward aglycones and that it is possible to create new polyene analogues with altered aglycones through genetic engineering. Inactivation of the DH11 domain of PKS led to novel polyene derivatives with mycosamine added to the altered polyketide backbones, further confirming the loose substrate specificity of polyene GTs. Furthermore, mutation of Ser346, Ser361, His362 or Cys387 of FscMI by site-directed mutagenesis significantly reduced its catalytic activity. Further analysis suggested that Ser361 and Cys387 are likely the critical donor interacting residues that could affect the activity of GT FscMI. To our knowledge, this is the first report of the critical residues in a polyene GT.
Starting Page	422
Ending Page	430
Page Count	9
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	9
Issue Number	3
Journal	Molecular BioSystems
DOI	10.1039/c2mb25464f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Glycosyltransferase Candicidin Aglycone Streptomyces Glyceraldehyde 3-phosphate Macrolide Polyketide synthase Polyketide Gene Antibiotics Polyene Site-directed mutagenesis PKS Genetic engineering Mutation
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Initiation of polyene macrolide biosynthesis: interplay between polyketide synthase domains and modules as revealed via domain swapping, mutagenesis, and heterologous complementation.

Regulation by aromatic amino acids of the biosynthesis of candicidin by Streptomyces griseus.

Site-directed mutagenesis of glyceraldehyde-3-phosphate

New insights into polyene macrolide biosynthesis in Couchioplanes caeruleus

Active site residues governing substrate selectivity and polyketide chain length in aloesone synthase

Ecologıa, gene - tica y microbiologıa, a - rea de.

Amino acid-accepting ketosynthase domain from a trans-AT polyketide synthase exhibits high selectivity for predicted intermediate

Site-specific Mutagenesis and Domain Substitutions in the Loading Module of the Nystatin Polyketide Synthase, and Their Effects on Nystatin Biosynthesis in Streptomyces noursei

Oxazolomycin Biosynthesis in Streptomyces albus JA3453 Featuring an “Acyltransferase-less” Type I Polyketide Synthase That Incorporates Two Distinct Extender Units

In vivo investigation of the substrate recognition capability and activity affecting amino acid residues of glycosyltransferase FscMI in the biosynthesis of candicidin

Similar Documents

Initiation of polyene macrolide biosynthesis: interplay between polyketide synthase domains and modules as revealed via domain swapping, mutagenesis, and heterologous complementation.

Regulation by aromatic amino acids of the biosynthesis of candicidin by Streptomyces griseus.

Site-directed mutagenesis of glyceraldehyde-3-phosphate

New insights into polyene macrolide biosynthesis in Couchioplanes caeruleus

Active site residues governing substrate selectivity and polyketide chain length in aloesone synthase

Ecologıa, gene - tica y microbiologıa, a - rea de.

Amino acid-accepting ketosynthase domain from a trans-AT polyketide synthase exhibits high selectivity for predicted intermediate

Site-specific Mutagenesis and Domain Substitutions in the Loading Module of the Nystatin Polyketide Synthase, and Their Effects on Nystatin Biosynthesis in Streptomyces noursei

Oxazolomycin Biosynthesis in Streptomyces albus JA3453 Featuring an “Acyltransferase-less” Type I Polyketide Synthase That Incorporates Two Distinct Extender Units

In vivo investigation of the substrate recognition capability and activity affecting amino acid residues of glycosyltransferase FscMI in the biosynthesis of candicidin