NDLI: High resolution spatial mapping of brominated pyrrole-2-aminoimidazole alkaloids distributions in the marine sponge Stylissa flabellata via MALDI-mass spectrometry imaging

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High resolution spatial mapping of brominated pyrrole-2-aminoimidazole alkaloids distributions in the marine sponge Stylissa flabellata via MALDI-mass spectrometry imaging

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High resolution spatial mapping of brominated pyrrole-2-aminoimidazole alkaloids distributions in the marine sponge Stylissa flabellata via MALDI-mass spectrometry imaging

Content Provider	Royal Society of Chemistry (RSC)
Author	Welsh, David T. Carroll, Anthony R. Venter, Deon J. Pool, Gertruida F. Hamilton, Brett R. Yarnold, Jennifer E.
Copyright Year	2012
Abstract	A number of pharmacologically active brominated pyrrole-2-aminoimidazole (B-P-2-AI) alkaloids have been isolated from several families of marine sponges, including those belonging to the genus Stylissa. In the present study, MALDI mass spectrometry imaging (MALDI-imaging) was applied to determine the spatial distribution of B-P-2-AIs within 20 μm cross sections of S. flabellata. A number of previously characterised B-P-2-AIs were readily identified by MALDI-imaging and confirmed by MS–MS and NMR profiling. Unknown B-P-2-AIs were also observed. Discrete microchemical environments were revealed for several B-P-2-AIs including dibromophakellin which was localised within the external pinacoderm and internal network of choanoderm chambers. Additionally, dibromopalau'amine and konbu'acidin B were also found to be confined to the choanoderm, while sceptrin was found to be highly abundant within the mesohyl. Further brominated compounds of unknown structure were also observed to have distinct localisation in both choanoderm chambers and the pinacoderm. These findings provide insights into the chemical ecology of S. flabellata, as most B-P-2-AIs were found on highly exposed surfaces, where they may act to prevent pathogens, predation and/or biofouling. Moreover this study demonstrates the power of MALDI-imaging to visualise the location of a range of metabolites in situ and to characterise compounds by MS–MS directly from intact specimens without the need for extraction. These methodologies facilitate selective targeting of micro-regions of sponge to screen for symbiotic microbial candidates or genes that may be involved in the production of the correlated compounds, and may represent a change in paradigm for natural product drug development.
Starting Page	2249
Ending Page	2259
Page Count	11
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	8
Issue Number	9
Journal	Molecular BioSystems
DOI	10.1039/c2mb25152c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Symbiosis Mesohyl Mass spectrometry imaging Kombu Natural product Nuclear magnetic resonance spectroscopy Pinacoderm Chemical ecology Choanoderm Ionization Genus Biofouling Pyrrole Micrometre Sceptrin Sponge Artificial intelligence
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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