NDLI: Post-transcriptional Boolean computation by combining aptazymes controlling mRNA translation initiation and tRNA activation

Please wait, while we are loading the content...

High resolution spatial mapping of brominated pyrrole-2-aminoimidazole alkaloids distributions in the marine sponge Stylissa flabellata via MALDI-mass spectrometry imaging

Understanding microscopic binding of macrophage migration inhibitory factor with phenolic hydrazones by molecular docking, molecular dynamics simulations and free energy calculations

Dupuytren's disease metabolite analyses reveals alterations following initial short-term fibroblast culturing

Upregulated MALAT-1 contributes to bladder cancer cell migration by inducing epithelial-to-mesenchymal transition

Revisiting the role of the immunoproteasome in the activation of the canonical NF-κB pathway

Alterations of protein complexes and pathways in genetic information flow and response to stimulus contribute to Escherichia coli resistance to balofloxacin

Marked changes in red cell membrane proteins in hereditary spherocytosis: a proteomics approach

Molecular chaperones DnaK and DnaJ share predicted binding sites on most proteins in the E. coli proteome

Porphyrins from a metagenomic library of the marine sponge Discodermia calyx

Thresholding for biomarker selection in multivariate data using Higher Criticism

Construction of logic gates with the fluorene-based small molecule/DNA probes

Impaired JAK2-induced activation of STAT3 in failing human myocytes

Placenta proteome analysis from Down syndrome pregnancies for biomarker discovery

Prediction of chemical–protein interactions: multitarget-QSAR versus computational chemogenomic methods

Single-domain antibodies: a versatile and rich source of binders for breast cancer diagnostic approaches

Photocleavable peptide–oligonucleotide conjugates for protein kinase assays by MALDI-TOF MS

Characterization of a cold-adapted glutathione synthetase from the psychrophile Pseudoalteromonas haloplanktis

Inferring differences in the distribution of reaction rates across conditions

Interaction and photo-induced cleavage studies of a copper based chemotherapeutic drug with human serum albumin: spectroscopic and molecular docking study

The serum metabolomic study of intervention effects of the traditional Chinese medicine Shexiang Baoxin Pill and a multi-component medicine polypill in the treatment of myocardial infarction in rats

Back cover

Molecular BioSystems Vol - 8 Issue - 8

Molecular BioSystems Vol - 8 Issue - 7

Molecular BioSystems Vol - 8 Issue - 6

Molecular BioSystems Vol - 8 Issue - 5

Molecular BioSystems Vol - 8 Issue - 4

Molecular BioSystems Vol - 8 Issue - 3

Molecular BioSystems Vol - 8 Issue - 2

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Post-transcriptional Boolean computation by combining aptazymes controlling mRNA translation initiation and tRNA activation

Content Provider	Royal Society of Chemistry (RSC)
Author	Saragliadis, Athanasios Ausländer, Simon Wieland, Markus Hartig, Jörg S. Klauser, Benedikt Berthold, Michael R.
Copyright Year	2012
Abstract	In cellular systems environmental and metabolic signals are integrated for the conditional control of gene expression. On the other hand, artificial manipulation of gene expression is of high interest for metabolic and genetic engineering. Especially the reprogramming of gene expression patterns to orchestrate cellular responses in a predictable fashion is considered to be of great importance. Here we introduce a highly modular RNA-based system for performing Boolean logic computation at a post-transcriptional level in Escherichia coli. We have previously shown that artificial riboswitches can be constructed by utilizing ligand-dependent Hammerhead ribozymes (aptazymes). Employing RNA self-cleavage as the expression platform-mechanism of an artificial riboswitch has the advantage that it can be applied to control several classes of RNAs such as mRNAs, tRNAs, and rRNAs. Due to the highly modular and orthogonal nature of these switches it is possible to combine aptazyme regulation of activating a suppressor tRNA with the regulation of mRNA translation initiation. The different RNA classes can be controlled individually by using distinct aptamers for individual RNA switches. Boolean logic devices are assembled by combining such switches in order to act on the expression of a single mRNA. In order to demonstrate the high modularity, a series of two-input Boolean logic operators were constructed. For this purpose, we expanded our aptazyme toolbox with switches comprising novel behaviours with respect to the small molecule triggers thiamine pyrophosphate (TPP) and theophylline. Then, individual switches were combined to yield AND, NOR, and ANDNOT gates. This study demonstrates that post-transcriptional aptazyme-based switches represent versatile tools for engineering advanced genetic devices and circuits without the need for regulatory protein cofactors.
Starting Page	2242
Ending Page	2248
Page Count	7
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	8
Issue Number	9
Journal	Molecular BioSystems
DOI	10.1039/c2mb25091h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Escherichia Riboswitch RNA Escherichia coli Cofactor (biochemistry) Boolean algebra Hammerhead shark Orthogonality Protein Thiamine pyrophosphate Gene Genetic engineering Theophylline Transfer RNA
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Post-transcriptional Boolean computation by combining aptazymes controlling mRNA translation initiation and tRNA activation.

Riboswitch and small RNA modulate btuB translation initiation in Escherichia coli and trigger distinct mRNA regulatory mechanisms

5’UTR sequences influence protein levels in Escherichia coli by regulating translation initiation and mRNA stability

The mRNA of Human Cytoplasmic Arginyl-tRNA Synthetase Recruits Prokaryotic Ribosomes Independently

Regulation of the thiamine pyrophosphate (TPP)‐sensing riboswitch in NMT1 mRNA from Neurospora crassa

Selection of the mRNA translation initiation region by Escherichia coli ribosomes.

RNase T is responsible for the end-turnover of tRNA in Escherichia coli.

Post-transcriptional Defects of Antioxidant Selenoenzymes Cause Oxidative Stress under Methylmercury Exposure

Initiation of tRNA maturation by RNase E is essential for cell viability in E. coli.

Post-transcriptional Boolean computation by combining aptazymes controlling mRNA translation initiation and tRNA activation

Similar Documents

Post-transcriptional Boolean computation by combining aptazymes controlling mRNA translation initiation and tRNA activation.

Riboswitch and small RNA modulate btuB translation initiation in Escherichia coli and trigger distinct mRNA regulatory mechanisms

5’UTR sequences influence protein levels in Escherichia coli by regulating translation initiation and mRNA stability

The mRNA of Human Cytoplasmic Arginyl-tRNA Synthetase Recruits Prokaryotic Ribosomes Independently

Regulation of the thiamine pyrophosphate (TPP)‐sensing riboswitch in NMT1 mRNA from Neurospora crassa

Selection of the mRNA translation initiation region by Escherichia coli ribosomes.

RNase T is responsible for the end-turnover of tRNA in Escherichia coli.

Post-transcriptional Defects of Antioxidant Selenoenzymes Cause Oxidative Stress under Methylmercury Exposure

Initiation of tRNA maturation by RNase E is essential for cell viability in E. coli.

Post-transcriptional Boolean computation by combining aptazymes controlling mRNA translation initiation and tRNA activation