NDLI: The lipid composition of isolated cytoplasmic lipid droplets from a human cancer cell line, BE(2)M17

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Hypothesis: butyrate is not an HDAC inhibitor, but a product inhibitor of deacetylation

Proteoglycan sequence

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Mapping the genome of Plasmodium falciparum on the drug-like chemical space reveals novel anti-malarial targets and potential drug leads

Revisiting the homology modeling of G-protein coupled receptors: β₁-adrenoceptor as an example

The lipid composition of isolated cytoplasmic lipid droplets from a human cancer cell line, BE(2)M17

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The lipid composition of isolated cytoplasmic lipid droplets from a human cancer cell line, BE(2)M17

Content Provider	Royal Society of Chemistry (RSC)
Author	Wilson, Martin Arvanitis, Theodoros N. Shockcor, John P. Kauppinen, Risto A. Griffin, Julian L. Peet, Andrew C. Pan, Xiaoyan McConville, Carmel Brundler, Marie-Anne
Copyright Year	2012
Abstract	1H nuclear magnetic resonance spectroscopy (NMR) resonances from lipids in tumours are associated with tumour grade and treatment response. The origin of these NMR signals is mainly considered to be cytoplasmic lipid droplets (LDs). Techniques exist for isolating LDs but little is known about their composition and its relationship to NMR signals. In this work, density-gradient ultracentrifugation was performed on homogenised human cancer cells to isolate LDs. 1H NMR was performed on whole cells, isolated LDs and their extracts. Heteronuclear single quantum coherence spectroscopy (HSQC) and liquid chromatography mass spectroscopy (LC-MS) were performed on lipid extracts of LDs. Staining and microscopy were used to characterize isolated LDs. An excellent agreement in chemical shift and relative signal intensity was observed between lipid resonances in cells and isolated LD spectra supporting that NMR-visible lipids originate primarily from LDs. Isolated LDs showed high concentrations of unsaturated lipids, a oleic-to-linoleic acid ratio greater than two and a cholesteryl ester (ChE)-to-cholesterol (Ch) ratio close to unity. These ratios were several-fold greater than respective ratios in whole cells, demonstrating isolation is important to characterize LD composition. LDs contain a specific group of lipid species that are likely to contribute to the 1H NMR spectrum of cells.
Starting Page	1694
Ending Page	1700
Page Count	7
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	8
Issue Number	6
Journal	Molecular BioSystems
DOI	10.1039/c2mb05485j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Spectroscopy Chemical shift Microscopy Heteronuclear single quantum coherence spectroscopy Nuclear magnetic resonance spectroscopy Cholesteryl ester Nuclear magnetic resonance Differential centrifugation Lipid Cancer
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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The lipid composition of isolated cytoplasmic lipid droplets from a human cancer cell line, BE(2)M17

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