NDLI: Systematic analysis of the IgG antibody immune response against varicella zoster virus (VZV) using a self-assembled protein microarray

Content Provider	Royal Society of Chemistry (RSC)
Author	Sibani, Sahar Pothineni, Venkata Raveendra LaBaer, Joshua Campbell, Colin J. Haas, Jürgen Ceroni, Alessandro Baiker, Armin Bailer, Susanne M.
Copyright Year	2010
Abstract	Varicella zoster virus (VZV) is a human herpesvirus encoding at least 69 distinct viral proteins which causes chickenpox after primary infection and shingles during reactivation and which is particularly important in pregnancy and immunocompromised patients. Current serodiagnostic tests are either based on whole cell lysates or glycoprotein preparations. In order to investigate the humoral immune response to VZV infection or vaccination in more detail, and to improve the currently available diagnostic assays, we developed a nucleic acid programmable protein array (NAPPA) containing all 69 VZV proteins and performed a detailed analysis of 68 sera from individuals with either no, a previous or an acute VZV infection. In addition to the known reactive glycoprotein antigens (ORF 5, ORF 14, ORF 31, ORF 37, ORF 68), we discovered IgG antibodies against a variety of other membrane (ORF 2, ORF 24), capsid (ORF 20, ORF 23, ORF 43) and tegument (ORF 53, ORF 9, ORF 11) proteins, as well as other proteins involved in virus replication and assembly (ORF 25, ORF 26, ORF 28) and the transactivator proteins ORF 12, ORF 62 and ORF 63. All of these antigens were only reactive in a subset of VZV-positive individuals. A subset of the newly identified VZV antigens was validated by western blot analysis. Using these seroreactive new VZV antigens, more sensitive assays and tests distinguishing between different clinical entities may be developed.
Starting Page	1604
Ending Page	1610
Page Count	7
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	6
Issue Number	9
Journal	Molecular BioSystems
DOI	10.1039/c003798b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Varicella zoster virus Western blot ORF Viral tegument Antibody Herpesviridae Immunoglobulin G Chickenpox Immunodeficiency Glycoprotein ORF 2 Protein Open reading frame Virus Capsid
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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Diagnostic Usefulness of Varicella Zoster Virus-Specific Immunoglobulin (Ig) A and IgG in Patients With Herpes Zoster.

Humoral and cellular immunity to varicella-zoster virus glycoprotein gpi and to a non-glycosylated protein, p170, in the strain 2 guinea-pig (1987).

Investigation of varicella-zoster virus-infected cell proteins that elicit antibody production during primary varicella using the immune transfer method (1984).

Virion association of IE62, the varicella-zoster virus (VZV) major transcriptional regulatory protein, requires expression of the VZV open reading frame 66 protein kinase

Protein kinase (1993).

2000. Nuclear accumulation of IE62, the varicella-zoster virus (VZV) major transcriptional regulatory protein, is inhibited by phosphorylation mediated by the VZV open reading frame 66 protein kinase

Absence of varicella-zoster virus (vzv) glycoprotein v does not alter growth of vzv (1994).

Varicella-zoster virus open reading frame 1 encodes a membrane protein that is dispensable for growth of vzv in vitro. Virology 206:835–842 (1995).

Antibody response to varicella-zoster vi us surface glycoproteins in chickenpox and shingles (1985)

Systematic analysis of the IgG antibody immune response against varicella zoster virus (VZV) using a self-assembled protein microarray

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Diagnostic Usefulness of Varicella Zoster Virus-Specific Immunoglobulin (Ig) A and IgG in Patients With Herpes Zoster.

Humoral and cellular immunity to varicella-zoster virus glycoprotein gpi and to a non-glycosylated protein, p170, in the strain 2 guinea-pig (1987).

Investigation of varicella-zoster virus-infected cell proteins that elicit antibody production during primary varicella using the immune transfer method (1984).

Virion association of IE62, the varicella-zoster virus (VZV) major transcriptional regulatory protein, requires expression of the VZV open reading frame 66 protein kinase

Protein kinase (1993).

2000. Nuclear accumulation of IE62, the varicella-zoster virus (VZV) major transcriptional regulatory protein, is inhibited by phosphorylation mediated by the VZV open reading frame 66 protein kinase

Absence of varicella-zoster virus (vzv) glycoprotein v does not alter growth of vzv (1994).

Varicella-zoster virus open reading frame 1 encodes a membrane protein that is dispensable for growth of vzv in vitro. Virology 206:835–842 (1995).

Antibody response to varicella-zoster vi us surface glycoproteins in chickenpox and shingles (1985)

Systematic analysis of the IgG antibody immune response against varicella zoster virus (VZV) using a self-assembled protein microarray