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The distribution profile and oxidation states of biometals in APP transgenic mouse brain: dyshomeostasis with age and as a function of the development of Alzheimer's disease

Effects of manganese and arsenic species on the level of energy related nucleotides in human cells

Proteomic analysis of ovarian cancer cell responses to cytotoxic gold compounds

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Metallomics Vol - 4 Issue - 2

Metallomics Vol -3

Metallomics Vol -2

Metallomics Vol -1

Effects of manganese and arsenic species on the level of energy related nucleotides in human cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Karst, Uwe Lohren, Hanna Humpf, Hans-Ulrich Bornhorst, Julia Ebert, Franziska Schwerdtle, Tanja
Copyright Year	2012
Abstract	Cellular adenine and pyridine nucleotides play important roles in the cellular energy and redox state. An imbalance in the cellular levels of these tightly regulated energy related nucleotides can lead to oxidative stress and thus is discussed to contribute to neurotoxic and carcinogenic processes. Here we established a reliable ion-pair reversed phase HPLC based method for the parallel quantification of six energy related nucleotides (ATP, ADP, ADP-ribose, AMP, NAD+, NADH) in cells and subsequently applied it to determine effects of manganese and arsenic species in cultured human cells. In human lung cells, MnCl2 (≥50 μM) decreased the levels of ATP, NAD+ and NADH as well as the NAD+/NADH ratio. This reflects a decline in the cellular energy metabolism, most likely resulting from a disturbance of the mitochondrial function. In contrast, cultured astrocytes were more resistant towards manganese. Regarding the arsenicals, a disturbance of the cellular energy related nucleotides was detected in lung cells for arsenite (≥50 μM), monomethylarsonous (≥1 μM), dimethylarsinous (≥1 μM) and dimethylarsinic acid (≥100 μM). Thereby, the single arsenicals seem to disturb the cellular energy and redox state by different mechanisms. Taken together, this study provides further evidence that cellular energy related nucleotides serve as sensitive indicators for toxic species exposure. When searching for a molecular mechanism of toxic compounds, the data illustrate the necessity of quantifying several energy related nucleotides in parallel, especially since ATP depletion, redox state alterations and oxidative stress are known to potentiate each other.
Starting Page	297
Ending Page	306
Page Count	10
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	4
Issue Number	3
Journal	Metallomics
DOI	10.1039/c2mt00164k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Oxidative stress Adenosine monophosphate AMP Arsenic Lung Adenine Redox Astrocyte High-performance liquid chromatography Metabolism ADP Adenosine diphosphate ribose Neurotoxicity NADH Pyridine Nicotinamide adenine dinucleotide ATP Arsenite Adenosine triphosphate Manganese
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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