NDLI: Inhibition of the purified 20S proteasome by non-heme iron complexes

Please wait, while we are loading the content...

Cu(ii)- and disulfide bonds-induced stabilization during the guanidine hydrochloride- and thermal-induced denaturation of NAD-glycohydrolase from the venom of Agkistrodon acutus

Inhibition of the purified 20S proteasome by non-heme iron complexes

Implication of cysteine residues in the selection of oxorhenium inhibitors of cyclophilin hCyp18

DNA binding and nucleolytic properties of Cu(ii) complexes of salicylaldehyde semicarbazones

Mutation in the CPC motif-containing 6th transmembrane domain affects intracellular localization, trafficking and copper transport efficiency of ATP7A protein in mosaic mutant mice––an animal model of Menkes disease

Design, synthesis, characterization and DNA-binding studies of a triphenyltin(iv) complex of N-glycoside (GATPT), a sugar based apoptosis inducer: in vitro and in vivo assessment of induction of apoptosis by GATPT

Synthesis, X-ray crystal structure, DNA binding, antioxidant and cytotoxicity studies of Ni(ii) and Pd(ii) thiosemicarbazone complexes

Inhibition of the purified 20S proteasome by non-heme iron complexes

Content Provider	Royal Society of Chemistry (RSC)
Author	Dou, Q. Ping Schmitt, Sara M. Kodanko, Jeremy J. Prakash, Jai
Copyright Year	2012
Abstract	Polypyridyl pentadentate ligands N4Py (1) and Bn-TPEN (2), along with their respective iron complexes, have been investigated for their ability to inhibit the purified 20S proteasome. Results demonstrated that the iron complexes of both ligands are potent inhibitors of the 20S proteasome (IC50 = 9.2 μM for [FeII(OH2)(N4Py)]2+ (3) and 4.0 μM for [FeII(OH2)(Bn-TPEN)]2+ (4)). Control experiments showed that ligand 1 or FeII alone showed no inhibition, whereas 2 was moderately active (IC50 = 96 μM), suggesting that iron, when bound to these ligands, plays a key role in proteasome inhibition. Results from time-dependent inactivation studies suggest different modes of action for the iron complexes. Time-dependent decay of proteasome activity was observed upon incubation in the presence of 4, which accelerated in the presence of DTT, suggesting reductive activation of O2 and oxidation of the 20S proteasome as a mode of action. In contrast, loss of 20S proteasome activity was not observed with 3 over time, suggesting inhibition through direct binding of the iron complex to the enzyme. Inhibition of the 20S proteasome by 4 was not blocked by reactive oxygen species scavengers, consistent with a unique oxidant being responsible for the time-dependent inhibition observed.
Starting Page	174
Ending Page	178
Page Count	5
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	4
Issue Number	2
Journal	Metallomics
DOI	10.1039/c2mt00131d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Oxygen Reactive oxygen species DTT Ligand Enzyme Digital terrestrial television Direct binding IC50 Proteasome
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Inhibition of the purified 20S proteasome by non-heme iron complexes

Copper binding modulates the platination of human copper chaperone Atox1 by antitumor trans-platinum complexes

Selective anticancer copper(ii)-mixed ligand complexes: targeting of ROS and proteasomes

In vitro heme and non-heme iron capture from hemoglobin, myoglobin and ferritin by bovine lactoferrin and implications for suppression of reactive oxygen species in vivo

Fe–oxy adducts of heme–Aβ and heme–hIAPP complexes: intermediates in ROS generation

Bacterial iron–sulfur cluster sensors in mammalian pathogens

Mammalian metallothioneins: properties and functions

Inhibitory effects of NAMI-A-like ruthenium complexes on prion neuropeptide fibril formation

Iron homeostasis and management of oxidative stress response in bacteria

Inhibition of the purified 20S proteasome by non-heme iron complexes

Similar Documents

Inhibition of the purified 20S proteasome by non-heme iron complexes

Copper binding modulates the platination of human copper chaperone Atox1 by antitumor trans-platinum complexes

Selective anticancer copper(ii)-mixed ligand complexes: targeting of ROS and proteasomes

In vitro heme and non-heme iron capture from hemoglobin, myoglobin and ferritin by bovine lactoferrin and implications for suppression of reactive oxygen species in vivo

Fe–oxy adducts of heme–Aβ and heme–hIAPP complexes: intermediates in ROS generation

Bacterial iron–sulfur cluster sensors in mammalian pathogens

Mammalian metallothioneins: properties and functions

Inhibitory effects of NAMI-A-like ruthenium complexes on prion neuropeptide fibril formation

Iron homeostasis and management of oxidative stress response in bacteria

Inhibition of the purified 20S proteasome by non-heme iron complexes