NDLI: Differential transition metal uptake and fluorescent probe localization in hippocampal slices

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Metallomics Vol - 3 Issue - 7

Metallomics Vol - 3 Issue - 6

Metallomics Vol - 3 Issue - 5

Metallomics Vol - 3 Issue - 4

Metallomics Vol - 3 Issue - 3

Metallomics Vol - 3 Issue - 2

Metallomics Vol -2

Metallomics Vol -1

Differential transition metal uptake and fluorescent probe localization in hippocampal slices

Content Provider	Royal Society of Chemistry (RSC)
Author	Kay, Alan R. Rumschik, Sean M.
Copyright Year	2011
Abstract	Metals are taken up by the combined action of metal transporters and ion channels. In this communication we have measured the uptake of the biologically important transition metals Mn, Fe, Co, Ni, Cu, Zn and Cd by rat and mouse hippocampal slices using the fluorescent probes FluoZin-3 (FZ3) and Newport Green (NPG), introduced by acetoxymethyl ester (AM) loading. The combination of metals and probes is also used to attempt to localize cellular sites into which metals translocate. We show that FZ3 and NPG partition into different cellular compartments; FZ3 into neuropil, whereas NPG localizes in neuropil and compartments within the cell bodies of neurons. Ni, Zn and Cd pass across the plasma membrane and then accumulate in intracellular vesicles and within intracellular membranes of cell bodies. The latter accumulate Cd, while synaptic vesicles take up Co. The passage of Mn, Cu and Fe into cells can be detected but there is some uncertainty about their disposition within the cell. All of our experiments are consistent with metals accumulating in intracellular compartments rather than the cytoplasm. Whether and to what extent there are transient elevations of free zinc levels in the cytoplasm remains unclear.
Starting Page	829
Ending Page	837
Page Count	9
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	3
Issue Number	8
Journal	Metallomics
DOI	10.1039/c1mt00024a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	NPG Cell membrane Ion Amplitude modulation Neuropil Zinc Ester Cu Zn Newport Green Mouse Newport RFC Cytoplasm
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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