NDLI: Towards the role of metal ions in the structural variability of proteins: Cd<sup>II</sup> speciation of a metal ion binding loop motif

Please wait, while we are loading the content...

Combination of PAGE and LA-ICP-MS as an analytical workflow in metallomics: state of the art, new quantification strategies, advantages and limitations

Subcellular localization of a fluorescent derivative of Cu^II(atsm) offers insight into the neuroprotective action of Cu^II(atsm)

Fast and low sample consuming quantification of manganese in cell nutrient solutions by flow injection ICP-QMS

Titanium preferential binding sites in human serum transferrin at physiological concentrations

Stability assessment of different chelating moieties used for elemental labeling of bio-molecules

Phosphoglycerate mutase from Trypanosoma brucei is hyperactivated by cobalt in vitro, but not in vivo

Mechanistic studies on two dinuclear organogold(iii) compounds showing appreciable antiproliferative properties and a high redox stability

Exploring the microbial metalloproteome using MIRAGE

Towards the role of metal ions in the structural variability of proteins: Cd^II speciation of a metal ion binding loop motif

Assessment of willow (Salix sp.) as a woody heavy metal accumulator: field survey and in vivo X-ray analyses

Direct analysis and stability of methylated trivalent arsenic metabolites in cells and tissues

Evaluation of heavy metal toxicity in eukaryotes using a simple functional assay

Molecular operation of metals into the function and state of photosystem II

The preferential accumulation of cadmium in the head portion of the freshwater planarian, Dugesia japonica (Platyhelminthes: Turbellaria)

The influence of calcium ions on nickel modulation of NMDA receptor currents

Back matter

Back cover

Metallomics Vol - 3 Issue - 9

Metallomics Vol - 3 Issue - 8

Metallomics Vol - 3 Issue - 7

Metallomics Vol - 3 Issue - 6

Metallomics Vol - 3 Issue - 5

Metallomics Vol - 3 Issue - 4

Metallomics Vol - 3 Issue - 3

Metallomics Vol - 3 Issue - 2

Metallomics Vol -2

Metallomics Vol -1

Towards the role of metal ions in the structural variability of proteins: Cd^II speciation of a metal ion binding loop motif

Content Provider	Royal Society of Chemistry (RSC)
Author	Szunyogh, Dániel Christensen, Niels Johan Larsen, Flemming H. Thulstrup, Peter W. Jancsó, Attila Gyurcsik, Béla Hemmingsen, Lars
Copyright Year	2011
Abstract	A de novo designed dodecapeptide (HS), inspired by the metal binding loops of metal-responsive transcriptional activators, was synthesized. The aim was to create a model system for structurally promiscuous and intrinsically unstructured proteins, and explore the effect of metal ions on their structure and dynamics. The interaction with CdII was investigated by UV, synchrotron radiation CD, 1H NMR, and perturbed angular correlation (PAC) of γ-rays spectroscopy, pH-potentiometry, and molecular modelling. The peptide mainly displays characteristics of random coil in the CD spectra, and the molecular dynamics simulations demonstrate that it is unstructured with transient and varying helical content. The spectroscopic studies revealed the formation of loop structures with the coordination of the two Cys-thiolates close to each end of the HSpeptide, in the presence of one equivalent of CdII per ligand. The imidazole moiety from histidine is also bound to CdII at neutral pH and above. In the presence of 0.5 equivalent of CdII per HS metal bridged structures with e.g.CdS2N2 and possibly CdS4 coordination geometries are formed above pH ∼6. In an equilibrium of several co-existing species the peptide is exchanging between a number of structures also in its metal ion bound state(s), as indicated by NMR and PAC data.
Starting Page	1331
Ending Page	1339
Page Count	9
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	3
Issue Number	12
Journal	Metallomics
DOI	10.1039/c1mt00138h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Spectroscopy Ligand Molecular dynamics Ion PAC Molecular modelling Histidine Intrinsically disordered proteins Imidazole PH Nuclear magnetic resonance Random coil Peptide Synchrotron radiation
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Metal ion mediated transition from random coil to β-sheet and aggregation of Bri2-23, a natural inhibitor of Aβ aggregation

PtCl₂(phen) disrupts the metal ions binding to amyloid-β peptide

The role of His-50 of α-synuclein in binding Cu(ii): pH dependence, speciation, thermodynamics and structure

pH-regulated metal–ligand switching in the HM loop of ATP7A: a new paradigm for metal transfer chemistry

The large intracellular loop of hZIP4 is an intrinsically disordered zinc binding domain†

Role of metal ions in aggregation of intrinsically disordered proteins in neurodegenerative diseases.

Molecular Transfer Model for pH effects on Intrinsically Disordered Proteins: Theory and Applications

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Synchrotron radiation for direct analysis of metalloproteins on electrophoresis gels

Towards the role of metal ions in the structural variability of proteins: Cd^II speciation of a metal ion binding loop motif

Similar Documents

Metal ion mediated transition from random coil to β-sheet and aggregation of Bri2-23, a natural inhibitor of Aβ aggregation

PtCl2(phen) disrupts the metal ions binding to amyloid-β peptide

The role of His-50 of α-synuclein in binding Cu(ii): pH dependence, speciation, thermodynamics and structure

pH-regulated metal–ligand switching in the HM loop of ATP7A: a new paradigm for metal transfer chemistry

The large intracellular loop of hZIP4 is an intrinsically disordered zinc binding domain†

Role of metal ions in aggregation of intrinsically disordered proteins in neurodegenerative diseases.

Molecular Transfer Model for pH effects on Intrinsically Disordered Proteins: Theory and Applications

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Synchrotron radiation for direct analysis of metalloproteins on electrophoresis gels

Towards the role of metal ions in the structural variability of proteins: CdII speciation of a metal ion binding loop motif

PtCl₂(phen) disrupts the metal ions binding to amyloid-β peptide

Towards the role of metal ions in the structural variability of proteins: Cd^II speciation of a metal ion binding loop motif