NDLI: In vivo bioavailability of selenium in enriched Pleurotus ostreatus mushrooms

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Simultaneous multiple element detection by particle beam/hollow cathode-optical emission spectroscopy as a tool for metallomic studies: Determinations of metal binding with apo-transferrin

In vivo bioavailability of selenium in enriched Pleurotus ostreatus mushrooms

Formation of methylselenol, dimethylselenide and dimethyldiselenide in in vitro metabolism models determined by headspace GC-MS

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Metallomics Vol -1

In vivo bioavailability of selenium in enriched Pleurotus ostreatus mushrooms

Content Provider Royal Society of Chemistry (RSC)
Author Naozuka, Juliana Silva, Marliane C. S. da Oliveira, Pedro V. Kasuya, Maria C. M. Costa, Neuza M. B. Vanetti, Maria C. D. Bazzolli, Denise M. S.
Copyright Year 2010
Abstract The in vivobioavailability of Se was investigated in enriched Pleurotus ostreatus mushrooms. A bioavailability study was performed using 64 Wistar male rats separated in 8 groups and fed with different diets: without Se, with mushrooms without Se, with enriched mushrooms containing 0.15, 0.30 or 0.45 mg kg−1Se and a normal diet containing 0.15 mg kg−1 of Se using sodium selenate. The experiment was performed in two periods: depletion (14 days) and repletion (21 days), according to the Association of Official Analytical Chemists. After five weeks, the rats were sacrificed under carbon dioxide, and blood was drawn by heart puncture. Blood plasma was separated by centrifugation. The total Se concentration in the plasma of rats fed with enriched mushrooms was higher than in rats fed with a normal diet containing sodium selenate. The plasma protein profiles were obtained using size exclusion chromatography (SEC) and UV detectors. Aliquots of effluents (0.5 mL per minute) were collected throughout in the end of the chomatographic column. However, Se was determined by graphite furnace atomic absorption spectrometry (GF AAS) only in the aliquots where proteins were detected by SEC-UV. The plasma protein profile of rats fed with different diets was similar. The highest Se concentration was observed in a peptide presenting 8 kDa. Furthermore, the higher Se concentration in this peptide was obtained for rats fed with a diet using enriched mushrooms (7 μg L−1Se) compared to other diets (2–5 μg L−1Se). These results showed that Se-enriched mushrooms can be considered as an alternative Se food source for humans, due to their high bioavailability.
Starting Page 162
Ending Page 166
Page Count 5
File Format HTM / HTML PDF
ISSN 17565901
Volume Number 2
Issue Number 2
Journal Metallomics
DOI 10.1039/b915780h
Language English
Publisher Royal Society of Chemistry
Access Restriction Open
Subject Keyword 21 Days Centrifugation Carbon dioxide Selenate Graphite furnace atomic absorption Bioavailability Blood plasma SEC Pleurotus Sodium Association of Official Analytical Chemists Size-exclusion chromatography U.S. Securities and Exchange Commission Peptide Blood proteins
Content Type Text
Resource Type Article
Subject Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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Iron bioavailability from cereal products enriched with Pleurotus ostreatus mushrooms in rats with induced anaemia.

Sequential extractions of selenium soils from Stewart Lake: total selenium and speciation measurements with ICP-MS detection

Identification and characterization of selenium species in enriched green onion (Allium fistulosum) by HPLC-ICP-MS and ESI-ITMS

The Main Structural Unit Elucidation and Immunomodulatory Activity In Vitro of a Selenium-Enriched Polysaccharide Produced by Pleurotus ostreatus

FORTIFICATION OF EDIBLE MUSHROOMS (Pleurotus ostreatus) WITH CALCIUM, SELENIUM AND VITAMIN C

FORTIFICACIÓN DE HONGOS COMESTIBLES (Pleurotus ostreatus) CON CALCIO, SELENIO Y VITAMINA C FORTIFICATION OF EDIBLE MUSHROOMS (Pleurotus ostreatus) WITH CALCIUM, SELENIUM AND VITAMIN C

Cytoprotective effect of selenium polysaccharide from Pleurotus ostreatus against H2O2-induced oxidative stress and apoptosis in PC12 cells

The effect of carbon dioxide on the quality of the mushrooms

Assessment and comparison of selenium-enriched maize with sodium selenite and sodium selenate

In vivo bioavailability of selenium in enriched Pleurotus ostreatus mushrooms

Content Provider	Royal Society of Chemistry (RSC)
Author	Naozuka, Juliana Silva, Marliane C. S. da Oliveira, Pedro V. Kasuya, Maria C. M. Costa, Neuza M. B. Vanetti, Maria C. D. Bazzolli, Denise M. S.
Copyright Year	2010
Abstract	The in vivobioavailability of Se was investigated in enriched Pleurotus ostreatus mushrooms. A bioavailability study was performed using 64 Wistar male rats separated in 8 groups and fed with different diets: without Se, with mushrooms without Se, with enriched mushrooms containing 0.15, 0.30 or 0.45 mg kg−1Se and a normal diet containing 0.15 mg kg−1 of Se using sodium selenate. The experiment was performed in two periods: depletion (14 days) and repletion (21 days), according to the Association of Official Analytical Chemists. After five weeks, the rats were sacrificed under carbon dioxide, and blood was drawn by heart puncture. Blood plasma was separated by centrifugation. The total Se concentration in the plasma of rats fed with enriched mushrooms was higher than in rats fed with a normal diet containing sodium selenate. The plasma protein profiles were obtained using size exclusion chromatography (SEC) and UV detectors. Aliquots of effluents (0.5 mL per minute) were collected throughout in the end of the chomatographic column. However, Se was determined by graphite furnace atomic absorption spectrometry (GF AAS) only in the aliquots where proteins were detected by SEC-UV. The plasma protein profile of rats fed with different diets was similar. The highest Se concentration was observed in a peptide presenting 8 kDa. Furthermore, the higher Se concentration in this peptide was obtained for rats fed with a diet using enriched mushrooms (7 μg L−1Se) compared to other diets (2–5 μg L−1Se). These results showed that Se-enriched mushrooms can be considered as an alternative Se food source for humans, due to their high bioavailability.
Starting Page	162
Ending Page	166
Page Count	5
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	2
Issue Number	2
Journal	Metallomics
DOI	10.1039/b915780h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	21 Days Centrifugation Carbon dioxide Selenate Graphite furnace atomic absorption Bioavailability Blood plasma SEC Pleurotus Sodium Association of Official Analytical Chemists Size-exclusion chromatography U.S. Securities and Exchange Commission Peptide Blood proteins
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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