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The Main Structural Unit Elucidation and Immunomodulatory Activity In Vitro of a Selenium-Enriched Polysaccharide Produced by Pleurotus ostreatus
| Content Provider | MDPI |
|---|---|
| Author | Wang, De Wang, Jiahui Liu, Hui Liu, Meng Yang, Yanjing Zhong, Shian |
| Copyright Year | 2022 |
| Description | In recent years, the structure of selenium-enriched polysaccharides and their application in immunomodulation have attracted much attention. In previous studies, we extracted and purified a novel selenium-enriched Pleurotus ostreatus polysaccharide called Se-POP-21, but its structure and immunomodulatory activity were still unclear. In this study, the main structural unit formula of Se-POP-21 was characterized by methylation analysis and an NMR experiment. The results showed that the backbone of Se-POP-21 was $→[2,6)-α-D-Galp-(1→6)-α-D-Galp-(1]_{4}→2,4)-β-L-Arap-(1→[2,6)-α-D-Galp-(1→6)-α-D-Galp-(1]_{4}$→, branched chain of β-D-Manp-(1→ and β-D-Manp-(1→4)-β-L-Arap-(1→ connected with →2,6)-α-D-Galp-(1→ and →2,4)-β-L-Arap-(1→,respectively, through the O-2 bond. In vitro cell experiments indicated that Se-POP-21 could significantly enhance the proliferation and phagocytosis of RAW264.7 cells, upregulate the expression of costimulatory molecules CD80/CD86, and promote RAW264.7 cells to secrete NO, ROS, TNF-α, IL-1β, and IL-6 by activating the NF-κB protein. The results of this study indicate that Se-POP-21 can effectively activate RAW264.7 cells. Thus, it has the potential to be used in immunomodulatory drugs or functional foods. |
| Starting Page | 2591 |
| e-ISSN | 14203049 |
| DOI | 10.3390/molecules27082591 |
| Journal | Molecules |
| Issue Number | 8 |
| Volume Number | 27 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-04-18 |
| Access Restriction | Open |
| Subject Keyword | Molecules Integrative and Complementary Medicine Pleurotus Ostreatus Selenium-enriched Polysaccharides Immunomodulatory Activity |
| Content Type | Text |
| Resource Type | Article |
