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Metallomics Vol - 7 Issue - 8

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The large intracellular loop of hZIP4 is an intrinsically disordered zinc binding domain

Content Provider	Royal Society of Chemistry (RSC)
Author	Nguyen, Tuong-Vi Dzul, Stephen P. Dempski, Robert E. Doyon, Brian Stemmler, Timothy L. Bafaro, Elizabeth M. Antala, Sagar
Copyright Year	2015
Abstract	The human (h) ZIP4 transporter is a plasma membrane protein which functions to increase the cytosolic concentration of zinc. hZIP4 transports zinc into intestinal cells and therefore has a central role in the absorption of dietary zinc. hZIP4 has eight transmembrane domains and encodes a large intracellular loop between transmembrane domains III and IV, M3M4. Previously, it has been postulated that this domain regulates hZIP4 levels in the plasma membrane in a zinc-dependent manner. The objective of this research was to examine the zinc binding properties of the large intracellular loop of hZIP4. Therefore, we have recombinantly expressed and purified M3M4 and showed that this domain binds two zinc ions. Using a combination of site-directed mutagenesis, metal binding affinity assays, and X-ray absorption spectroscopy, we demonstrated that the two Zn2+ ions bind sequentially, with the first Zn2+ binding to a CysHis3 site with a nanomolar binding affinity, and the second Zn2+ binding to a His4 site with a weaker affinity. Circular dichroism spectroscopy revealed that the M3M4 domain is intrinsically disordered, with only a small structural change induced upon Zn2+ coordination. Our data supports a model in which the intracellular M3M4 domain senses high cytosolic Zn2+ concentrations and regulates the plasma membrane levels of the hZIP4 transporter in response to Zn2+ binding.
Starting Page	1319
Ending Page	1330
Page Count	12
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	7
Issue Number	9
Journal	Metallomics
DOI	10.1039/c5mt00066a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Absorption spectroscopy Spectroscopy Membrane protein Cell membrane Histidinol dehydrogenase Site-directed mutagenesis X-ray absorption spectroscopy Zinc Circular dichroism
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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