NDLI: A cell-laden microfluidic hydrogel

Content Provider	Royal Society of Chemistry (RSC)
Author	Rubin, Jamie Ling, Yibo Huang, Catherine Demirci, Utkan Deng, Yuting Khademhosseini, Ali Karp, Jeffrey M.
Copyright Year	2007
Abstract	The encapsulation of mammalian cells within the bulk material of microfluidic channels may be beneficial for applications ranging from tissue engineering to cell-based diagnostic assays. In this work, we present a technique for fabricating microfluidic channels from cell-laden agarose hydrogels. Using standard soft lithographic techniques, molten agarose was molded against a SU-8 patterned silicon wafer. To generate sealed and water-tight microfluidic channels, the surface of the molded agarose was heated at 71 °C for 3 s and sealed to another surface-heated slab of agarose. Channels of different dimensions were generated and it was shown that agarose, though highly porous, is a suitable material for performing microfluidics. Cells embedded within the microfluidic molds were well distributed and media pumped through the channels allowed the exchange of nutrients and waste products. While most cells were found to be viable upon initial device fabrication, only those cells near the microfluidic channels remained viable after 3 days, demonstrating the importance of a perfused network of microchannels for delivering nutrients and oxygen to maintain cell viability in large hydrogels. Further development of this technique may lead to the generation of biomimetic synthetic vasculature for tissue engineering, diagnostics, and drug screening applications.
Starting Page	756
Ending Page	762
Page Count	7
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	7
Issue Number	6
Journal	Lab on a Chip
DOI	10.1039/b615486g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	SU-8 photoresist Microchannel (microtechnology) Oxygen Biomimetics Tissue engineering Wafer (electronics) Agarose Microfluidics
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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