NDLI: Electrical detection of germination of viable model Bacillus anthracis spores in microfluidic biochips

Please wait, while we are loading the content...

A 96-well microplate incorporating a replica molded microfluidic network integrated with photonic crystal biosensors for high throughput kinetic biomolecular interaction analysis

Multifunctional microvalves control by optical illumination on nanoheaters and its application in centrifugal microfluidic devices

One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device

Direct patterning of composite biocompatible microstructures using microfluidics

Geometrical optimization of helical flow in grooved micromixers

Continuous cytometric bead processing within a microfluidic device for bead based sensing platforms

Tuning microchannel wettability and fabrication of multiple-step Laplace valves

Electrical detection of germination of viable model Bacillus anthracis spores in microfluidic biochips

A parametric study of human fibroblasts culture in a microchannel bioreactor

Microparticle collection and concentration via a miniature surface acoustic wave device

Fluorescence spectrometer-on-a-fluidic-chip

An optofluidic device for surface enhanced Raman spectroscopy

Microfluidic pressure sensing using trapped air compression

A biological sensor platform using a pneumatic-valve controlled microfluidic device containing Tetrahymena pyriformis

Rapid fabrication of microchannels using microscale plasma activated templating (µPLAT) generated water molds

Glass microfabricated nebulizer chip for mass spectrometry

Pressure-driven spatiotemporal control of the laminar flow interface in a microfluidic network

Back matter

Back cover

Lab on a Chip Vol - 7 Issue - 4

Lab on a Chip Vol - 7 Issue - 3

Lab on a Chip Vol - 7 Issue - 2

Electrical detection of germination of viable model Bacillus anthracis spores in microfluidic biochips

Content Provider	Royal Society of Chemistry (RSC)
Author	Lim, Kwan-Seop Lee, S. W. Walter, T. M. Liu, Yi-Shao Yang, Liju Chang, Woo-Jin Bashir, R. Aronson, A.
Copyright Year	2007
Abstract	In this paper, we present a new impedance-based method to detect viable spores by electrically detecting their germination in real time within microfluidic biochips. We used Bacillus anthracis Sterne spores as the model organism. During germination, the spores release polar and ionic chemicals, such as dipicolinic acid (DPA), calcium ions, phosphate ions, and amino acids, which correspondingly increase the electrical conductivity of the medium in which the spores are suspended. We first present macro-scale measurements demonstrating that the germination of spores can be electrically detected at a concentration of 109 spores ml−1 in sample volumes of 5 ml, by monitoring changes in the solution conductivity. Germination was induced by introducing an optimized germinant solution consisting of 10 mM L-alanine and 2 mM inosine. We then translated these results to a micro-fluidic biochip, which was a three-layer device: one layer of polydimethylsiloxane (PDMS) with valves, a second layer of PDMS with micro-fluidic channels and chambers, and the third layer with metal electrodes deposited on a pyrex substrate. Dielectrophoresis (DEP) was used to trap and concentrate the spores at the electrodes with greater than 90% efficiency, at a solution flow rate of 0.2 μl min−1 with concentration factors between 107–109 spores ml−1, from sample volumes of 1–5 μl. The spores were captured by DEP in deionized water within 1 min (total volume used ranged from 0.02 μl to 0.2 μl), and then germinant solution was introduced to the flow stream. The detection sensitivity was demonstrated to be as low as about a hundred spores in 0.1 nl, which is equivalent to a macroscale detection limit of approximately 109 spores ml−1. We believe that this is the first demonstration of this application in microfluidic and BioMEMS devices.
Starting Page	603
Ending Page	610
Page Count	8
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	7
Issue Number	5
Journal	Lab on a Chip
DOI	10.1039/b702408h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Bio-MEMS Alanine Calcium Germination Model organism DEP Electrical resistivity and conductivity Sterne Phosphate Bacillus Inosine Pyrex Dielectrophoresis Molly McGuire Polydimethylsiloxane Purified water
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Bacillus cereus spores release alanine that synergizes with inosine to promote germination.

Effects of L-alanine and inosine germinants on the elasticity of Bacillus anthracis spores.

Bacillus cereus Spores Release Alanine that Synergizes with Inosine to Promote Germination

UV resistance of Bacillus anthracis spores revisited: validation of Bacillus subtilis spores as UV surrogates for spores of B. anthracis Sterne.

Spores of bacillus atrophaeus and bacillus anthracis sterne on nonreflective surfaces.

Identification of an in vivo inhibitor of bacillus anthracis spore germination *.

Anthrax Toxins in Context of Bacillus anthracis Spores and Spore Germination

Destruction of Bacillus anthracis strain Sterne 34F2 spores in postal envelopes by exposure to electron beam irradiation

Bacillus anthracis spores germinate extracellularly at air–liquid interface in an in vitro lung model under serum‐free conditions

Electrical detection of germination of viable model Bacillus anthracis spores in microfluidic biochips

Similar Documents

Bacillus cereus spores release alanine that synergizes with inosine to promote germination.

Effects of L-alanine and inosine germinants on the elasticity of Bacillus anthracis spores.

Bacillus cereus Spores Release Alanine that Synergizes with Inosine to Promote Germination

UV resistance of Bacillus anthracis spores revisited: validation of Bacillus subtilis spores as UV surrogates for spores of B. anthracis Sterne.

Spores of bacillus atrophaeus and bacillus anthracis sterne on nonreflective surfaces.

Identification of an in vivo inhibitor of bacillus anthracis spore germination *.

Anthrax Toxins in Context of Bacillus anthracis Spores and Spore Germination

Destruction of Bacillus anthracis strain Sterne 34F2 spores in postal envelopes by exposure to electron beam irradiation

Bacillus anthracis spores germinate extracellularly at air–liquid interface in an in vitro lung model under serum‐free conditions

Electrical detection of germination of viable model Bacillus anthracis spores in microfluidic biochips