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Bacillus anthracis spores germinate extracellularly at air–liquid interface in an in vitro lung model under serum‐free conditions
| Content Provider | Oxford Academic |
|---|---|
| Author | Powell, J.D. Hutchison, J.R. Hess, B.M. Straub, T.M. |
| Copyright Year | 2015 |
| Abstract | Aim: To better understand the parameters that govern spore dissemination after lung exposure using in vitro cell systems.Methods and ResultsWe evaluated the kinetics of uptake, germination and proliferation of Bacillus anthracis Sterne spores in association with human primary lung epithelial cells, Calu‐3 and A549 cell lines. We also analysed the influence of various cell culture medium formulations related to spore germination.ConclusionsWe found negligible spore uptake by epithelial cells, but germination and proliferation of spores in the serum‐free extracellular environment was evident. Spore germination was appreciably higher in immortalized cell cultures than in primary epithelial cells. Additionally, spores still germinated apically at a mucus‐secreting air–liquid interface lung barrier that was devoid of cell culture medium much earlier than medium‐only controls.Significance and Impact of the StudyThe role of lung epithelial cells in B. anthracis spore dissemination after inhalation remains poorly defined and rather controversial. These results are novel as they show spore germination is appreciably enhanced in the presence of lung cells in vitro, however, the cell line and cell state (air–liquid interface vs submerged in medium) dictates the extent of germination and in some cases proliferation. |
| Related Links | https://academic.oup.com/jambio/article-pdf/119/3/711/47324063/jambio0711.pdf |
| Ending Page | 723 |
| Starting Page | 711 |
| File Format | |
| ISSN | 13645072 |
| e-ISSN | 13652672 |
| DOI | 10.1111/jam.12872 |
| Journal | Journal of Applied Microbiology |
| Issue Number | 3 |
| Volume Number | 119 |
| Language | English |
| Publisher | Oxford Academic |
| Publisher Date | 2015-09-01 |
| Access Restriction | Open |
| Subject Keyword | Biological Sciences Microbiology Science and Mathematics Anthrax Bacillus Anthracis Germination Lung Epithelial Spore Sterne |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Applied Microbiology and Biotechnology Biotechnology |
