NDLI: Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Cho, Yee Sook Park, Je-Kyun Kim, Minseok S. Han, Yong-Mahn Han, Hyo-Won Kim, Janghwan
Copyright Year	2007
Abstract	We have developed a novel method for fabricating an embryonic stem cell divider (ESCD) constructed from a poly(dimethylsiloxane) (PDMS) replica with a square or hexagonal pattern, and have proposed a new dissociation method for human embryonic stem cells (ESCs). An aspect ratio of the device as high as 2 was perfectly replicated in the cutting line. Using the ESCD, human ESC colonies can be easily and efficiently dissociated into regular-sized ESC clumps without enzymatic treatment. The regularity of the ESC clumps dissociated by the ESCD was compared to that dissociated by a conventional mechanical method. Its quality and reliability were confirmed by maintaining undifferentiated ESCs up to the 15th passage. The ESCD will contribute to the advance quality control of in vitro ESC cultures and allow large-scale production of qualified ESCs with tremendous time- and work-saving.
Starting Page	513
Ending Page	515
Page Count	3
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	7
Issue Number	4
Journal	Lab on a Chip
DOI	10.1039/b617760n
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	ESC Stem cell Dissociative Aspect ratio Polydimethylsiloxane ESCD
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells