NDLI: A micro-scale multi-frequency reactance measurement technique to detect bacterial growth at low bio-particle concentrations

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Research Highlights ( Lab on a Chip Vol - 6 Issue - 5 )

Active transport: a new chemical separation method?

On-chip pressure injection for integration of infrared-mediated DNA amplification with electrophoretic separation

A microsystem for sensing and patterning oxidative microgradients during cell culture

Development and multiplexed control of latching pneumatic valves using microfluidic logical structures

A nanofabricated planar aperture as a mimic of the nerve–muscle contact during synaptogenesis

A method for dynamic system characterization using hydraulic series resistance

Characterization of a microscale cyclical electrical field flow fractionation system

Critical particle size for fractionation by deterministic lateral displacement

An effervescent reaction micropump for portable microfluidic systems

Design and fabrication of a multilayered polymer microfluidic chip with nanofluidic interconnects via adhesive contact printing

Immunosensing of Staphylococcus enterotoxin B (SEB) in milk with PDMS microfluidic systems using reinforced supported bilayer membranes (r-SBMs)

A micro-scale multi-frequency reactance measurement technique to detect bacterial growth at low bio-particle concentrations

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Lab on a Chip Vol - 6 Issue - 4

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Lab on a Chip Vol - 6 Issue - 2

A micro-scale multi-frequency reactance measurement technique to detect bacterial growth at low bio-particle concentrations

Content Provider	Royal Society of Chemistry (RSC)
Author	Chang, Hsueh-Chia Battigelli, David A. Sengupta, Shramik
Copyright Year	2006
Abstract	The technique described enables the user to detect the presence and proliferation of bacteria through an increase in the bulk capacitance (C) of the suspension, which is proportional to the bacteria count, at practical frequencies less than 1 MHz. The geometry of the micro-capillary design employed increases the bulk resistance (R) of the medium, thus increasing its RC time. This makes the measured reactance sensitive to changes in the bulk capacitance, which is usually masked by the much larger surface capacitance. The sensitivity is further enhanced by the existence of a minimum in the value of the reactance at a frequency proportional to the inverse medium RC time. The value of this reactance minimum and the frequency at which the minimum is recorded are dependent on the bacteria count and permit the detection of an initial concentration of ∼100 CFU ml−1 of E. coli within 3 hours of incubation, in comparison with the previous reported values of about 8 hours, with an initial load of 1000 CFU ml−1.
Starting Page	682
Ending Page	692
Page Count	11
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	6
Issue Number	5
Journal	Lab on a Chip
DOI	10.1039/b516274b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Geometry Electrical reactance CFU Escherichia coli Bacteria Capacitance The Technique
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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A micro-scale multi-frequency reactance measurement technique to detect bacterial growth at low bio-particle concentrations

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