NDLI: A microsystem for sensing and patterning oxidative microgradients during cell culture

Content Provider	Royal Society of Chemistry (RSC)
Author	Maharbiz, Michel M. Pinelis, Mikhail Bansal, Tushar Park, Jaehyun
Copyright Year	2006
Abstract	We present the design, modeling, fabrication and testing of a microsystem for the electrolytic patterning and sensing of oxidative microgradients within 1 × 1 mm2 area during cell culture. The system employs an array of microfabricated electrodes (3–40 µm in width) embedded in gas-permeable microchannels to generate precise doses of dissolved oxygen (ranging from 10 fmol O2 mm−2 s−1 to 100 nmol O2 mm−2 s−1) via electrolysis. The microgradients generated by different microelectrodes in the array can be superimposed to pattern multi-dimensional oxygen profiles not possible with other methods. We demonstrate the patterning, sensing and quantification of dissolved oxygen microgradients in the 0 to 40% dO2 range using this microsystem. Reactive oxygen species generation and dosing is also quantified. Lastly, we demonstrate how the microtechnology enables new types of experiments in three different cell culture models: localized hyperoxia-induced apoptosis in C2C12 myoblasts, dynamic aerotaxis assays of Bacillus subtilis, and studies of calcium release in an ischemia/re-oxygenation myoblast model.
Starting Page	611
Ending Page	622
Page Count	12
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	6
Issue Number	5
Journal	Lab on a Chip
DOI	10.1039/b516483d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Cell culture Oxygen Reactive oxygen species Calcium Myocyte Microchannel (microtechnology) Electrolysis Ischemia Microfabrication Micrometre Bacillus Taxis Microtechnology Apoptosis
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A parametric study of human fibroblasts culture in a microchannel bioreactor

Fabrication of a modular tissue construct in a microfluidic chip

Generation of alginate microfibers with a roller-assisted microfluidic system

Rapid microfabrication of solvent-resistant biocompatible microfluidic devices

Gelatin based microfluidic devices for cell culture

Microfluidic stickers for cell- and tissue-based assays in microchannels

Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells

Flow of microgel capsules through topographically patterned microchannels

Microchip-based cell analysis and clinical diagnosis system

A microsystem for sensing and patterning oxidative microgradients during cell culture

Similar Documents

A parametric study of human fibroblasts culture in a microchannel bioreactor

Fabrication of a modular tissue construct in a microfluidic chip

Generation of alginate microfibers with a roller-assisted microfluidic system

Rapid microfabrication of solvent-resistant biocompatible microfluidic devices

Gelatin based microfluidic devices for cell culture

Microfluidic stickers for cell- and tissue-based assays in microchannels

Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells

Flow of microgel capsules through topographically patterned microchannels

Microchip-based cell analysis and clinical diagnosis system

A microsystem for sensing and patterning oxidative microgradients during cell culture