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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Stoneman, Michael Fox, Michael Raicu, Valerică Zeng, Chaoyang |
| Copyright Year | 2009 |
| Abstract | We report an improved method for production of microfluidic device masters using two-photon photopolymerization of SU-8 negative photoresist, which relies on a two-photon microscope (TPM) commonly used in imaging of biological samples. The device masters serve as negative relief structures for polydimethylsiloxane-based microfluidic devices. We observed that not only did the two-photon excitation of the SU-8 photoresist initiate crosslinking of the material in the region of the focus of the near-infrared laser beam (as expected) but it also resulted in emission of fluorescence in the visible range. The detected emission of SU-8 photoresist undergoing two-photon excitation displayed a strong correlation with the size of the polymerized objects produced during the exposure; this allowed the progress of the microfluidic master production process to be monitored in real-time. We demonstrate the use of the fluorescence detection during two-photon photopolymerization in the production of microfluidic devices, which were designed to trap individual yeast cells to be imaged with the same TPM used for microfluidic master writing. |
| Starting Page | 819 |
| Ending Page | 827 |
| Page Count | 9 |
| File Format | HTM / HTML PDF |
| ISSN | 14730197 |
| Volume Number | 9 |
| Issue Number | 6 |
| Journal | Lab on a Chip |
| DOI | 10.1039/b816993d |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | SU-8 photoresist Yeast Two-photon excitation microscopy Laser Polymerization Photoresist |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering |
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