NDLI: Drug testing on 3D in vitro tissues trapped on a microcavity chip

Content Provider	Royal Society of Chemistry (RSC)
Author	Fischer, Michael Robitzki, Andrea A. Rothermel, Andrée Simon, Jan C. Kloß, Daniel
Copyright Year	2008
Abstract	Close to realistic responses to anti-cancer drugs are not adequately provided in monolayer or single cells assays. 3-dimensional multicellular cultures (spheroids) mimicking in vivo-like conditions are established as cell biological models for microtumors/metastases. For a non-invasive real-time monitoring of the electrical parameters of such spheroid cultures we designed, fabricated and tested a 3D multifunctional electrode-based microcavity array. In a non-adherent assay acute tests with tumor spheroids were done maintaining their spherical shape and cellular arrangement. The sensor chip with 15 individual square microcavities containing four gold electrodes each was used for impedance spectroscopy to analyze the tissue models in terms of morphological and structural changes. Cell type specific differences in the spectra and varying responses to several anti-tumor drugs were found. Further development of the prototype will provide a promising tool for the use in pharmacological high-throughput studies.
Starting Page	879
Ending Page	884
Page Count	6
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	8
Issue Number	6
Journal	Lab on a Chip
DOI	10.1039/b800394g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Spectroscopy Spheroid Metastasis Three-dimensional space Electrical impedance
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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