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Lab on a Chip Vol - 8 Issue - 4

Lab on a Chip Vol - 8 Issue - 3

Lab on a Chip Vol - 8 Issue - 2

A membrane-based microfluidic device for controlling the flux of platelet agonists into flowing blood

Content Provider	Royal Society of Chemistry (RSC)
Author	Diamond, Scott L. Neeves, Keith B.
Copyright Year	2008
Abstract	The flux of platelet agonists into flowing blood is a critical event in thrombosis and hemostasis. However, few in vitro methods exist for examining and controlling the role of platelet agonists on clot formation and stability under hemodynamic conditions. In this paper, we describe a membrane-based method for introducing a solute into flowing blood at a defined flux. The device consisted of a track-etched polycarbonate membrane reversibly sealed between two microfluidic channels; one channel contained blood flowing at a physiologically relevant shear rate, and the other channel contained the agonist(s). An analytical model described the solute flux as a function of the membrane permeability and transmembrane pressure. The model was validated using luciferase as a model solute for transmembrane pressures of 50–400 Pa. As a proof-of-concept, the weak platelet agonist ADP was introduced into whole blood flowing at 250 s−1 at three fluxes (1.5, 2.4, and 4.4 × 10−18 mol μm−2 s−1). Platelet aggregation was monitored by fluorescence microscopy during the experiment and the morphology of aggregates was determined by post hoc confocal and electron microscopy. At the lowest flux (1.5 × 10−18 mol μm−2 s−1), we observed little to no aggregation. At the higher fluxes, we observed monolayer (2.4 × 10−18 mol μm−2 s−1) and multilayer (4.4 × 10−18 mol μm−2 s−1) aggregates of platelets and found that the platelet density within an aggregate increased with increasing ADP flux. We expect this device to be a useful tool in unraveling the role of platelet agonists on clot formation and stability.
Starting Page	701
Ending Page	709
Page Count	9
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	8
Issue Number	5
Journal	Lab on a Chip
DOI	10.1039/b717824g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Electron microscope Agonist Luciferase Permeation Adenosine diphosphate Fluorescence microscope ADP Thrombosis Shear rate Platelet Hemostasis Micrometre Polycarbonate
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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