NDLI: Nanoparticle phosphate-based composites as vehicles for antimony delivery to macrophages: possible use in leishmaniasis

Please wait, while we are loading the content...

An integrin-targeting nanosystem as a carrier of the selenadiazole derivative to induce ROS-mediated apoptosis in bladder cancer cells, from rational design to action mechanisms

pH-Triggered burst intracellular release from hollow microspheres to induce autophagic cancer cell death

Reactive oxygen species (ROS) responsive PEG–PCL nanoparticles with pH-controlled negative-to-positive charge reversal for intracellular delivery of doxorubicin

Back cover

Journal of Materials Chemistry B Vol - 3 Issue - 39

Journal of Materials Chemistry B Vol - 3 Issue - 29

Journal of Materials Chemistry B Vol - 3 Issue - 19

Journal of Materials Chemistry B Vol - 3 Issue - 9

Journal of Materials Chemistry B Vol - 3 Issue - 8

Journal of Materials Chemistry B Vol - 3 Issue - 7

Journal of Materials Chemistry B Vol - 3 Issue - 6

Journal of Materials Chemistry B Vol - 3 Issue - 5

Journal of Materials Chemistry B Vol -2

Journal of Materials Chemistry B Vol -1

Nanoparticle phosphate-based composites as vehicles for antimony delivery to macrophages: possible use in leishmaniasis

Content Provider	Royal Society of Chemistry (RSC)
Author	Silva, José Bento Borba da Speziali, Nivaldo Lucio Junior, José Dias Corrêa Alvarenga, Betânia Mara Demicheli, Cynthia Frézard, Fréderic Melo, Maria Norma Gomes, Juliana Moreira Mendonça
Copyright Year	2015
Abstract	Pentavalent antimonial drugs such as N-methylglucamine antimonate (Glucantime®) are used for treating leishmaniasis but produce severe side effects, including cardiotoxicity and hepatotoxicity. We characterized the physicochemical properties of 3 nanoparticle phosphate-based composites (NPCs; NPC0, NPC3, and NPC5) as Sb(V) carriers for specifically targeting macrophages and reducing systemic side effects. NPCs were synthesized in liquid media and sterilized at 25 kGy before use. Macrophage viability and NPC toxicity, independent of Sb uptake, were evaluated to assess NPC safety in visceral leishmaniasis treatment. NPC zeta potential, conductivity, diameter, Sb content, and crystallinity were determined using electrophoretic light scattering, scanning electron microscopy (SEM), conductance, graphite furnace atomic absorption spectrometry (GFAAS), and X-ray diffraction, respectively. In vitro NPC cytotoxicity against murine peritoneal macrophages was evaluated using MTT assays, and Sb amounts internalized by macrophages were determined using GFAAS. The rate of macrophage infection caused by Leishmania infantum was assayed in vitro, by using Glucantime® as a reference drug. NPCs featured negative zeta potentials (−15.5 to −19.5 mV), mean diameters around 180 nm, and a low dissolution constant in Milli-Q water (<0.0197 mS cm−1), and were prepared using 0.0 (NPC0) to 36.2 μg mL−1 Sb (NPC5). NPC5 exhibited characteristic crystalline peaks resembling mopungite, but other NPCs exhibited predominantly amorphous structures. Cell viability was not markedly affected at any NPC concentration tested. Light microscopy, SEM, and GFAAS data revealed NPC internalization and intracellular Sb retention. Amastigote infection was reduced by both Sb-containing NPC3 and Sb-lacking NPC0, but NPC3 was more effective. These data indicate the potential of NPCs as Sb nanocarriers for specifically targeting macrophages and lowering Sb dosage without reducing leishmanicidal activity.
Starting Page	9250
Ending Page	9259
Page Count	10
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	3
Issue Number	48
Journal	Journal of Materials Chemistry B
DOI	10.1039/c5tb00376h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	NPC Pentavalent antimonial Leishmaniasis Cardiotoxicity Hepatotoxicity Nanoparticle Macrophage Gray (unit) Non-player character Zeta potential Electrophoretic light scattering Scanning electron microscope Graphite furnace atomic absorption X-ray crystallography Cytotoxicity Leishmania infantum Milli-Q Microscopy Amastigote
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Investigations on the Structures of the Pentavalent Antimonial Drugs for the Treatment of Leishmaniasis

Mixed Antimony(V) Complexes with Different Sugars to Modulate the Oral Bioavailability of Pentavalent Antimonial Drugs

Two small yellowish papules in a 1 year-old boy: cutaneous leishmaniasis

In Vitro Reduced Susceptibility to Pentavalent Antimonials of a Leishmania infantum Isolate from a Human Cutaneous Leishmaniasis Case in Central Italy

Efficacy of Spironolactone Treatment in Murine Models of Cutaneous and Visceral Leishmaniasis

Cross-resistance of Leishmania infantum isolates to nitric oxide from patients refractory to antimony treatment, and greater tolerance to antileishmanial responses by macrophages

An alternative antimonial schedule to be used in cutaneous leishmaniasis when high doses of antimony are undesirable

A Double-blind, Randomized Trial to Evaluate Miltefosine and Topical Granulocyte Macrophage Colony-stimulating Factor in the Treatment of Cutaneous Leishmaniasis Caused by Leishmania braziliensis in Brazil

Leishmania tropica–Induced Cutaneous and Presumptive Concomitant Viscerotropic Leishmaniasis With Prolonged Incubation

Nanoparticle phosphate-based composites as vehicles for antimony delivery to macrophages: possible use in leishmaniasis

Similar Documents

Investigations on the Structures of the Pentavalent Antimonial Drugs for the Treatment of Leishmaniasis

Mixed Antimony(V) Complexes with Different Sugars to Modulate the Oral Bioavailability of Pentavalent Antimonial Drugs

Two small yellowish papules in a 1 year-old boy: cutaneous leishmaniasis

In Vitro Reduced Susceptibility to Pentavalent Antimonials of a Leishmania infantum Isolate from a Human Cutaneous Leishmaniasis Case in Central Italy

Efficacy of Spironolactone Treatment in Murine Models of Cutaneous and Visceral Leishmaniasis

Cross-resistance of Leishmania infantum isolates to nitric oxide from patients refractory to antimony treatment, and greater tolerance to antileishmanial responses by macrophages

An alternative antimonial schedule to be used in cutaneous leishmaniasis when high doses of antimony are undesirable

A Double-blind, Randomized Trial to Evaluate Miltefosine and Topical Granulocyte Macrophage Colony-stimulating Factor in the Treatment of Cutaneous Leishmaniasis Caused by Leishmania braziliensis in Brazil

Leishmania tropica–Induced Cutaneous and Presumptive Concomitant Viscerotropic Leishmaniasis With Prolonged Incubation

Nanoparticle phosphate-based composites as vehicles for antimony delivery to macrophages: possible use in leishmaniasis