NDLI: Controlling performance of lipase immobilised on bioinspired silica

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Contents list

Calcium phosphate-based cements: clinical needs and recent progress

Gastric-floating microcapsules provide controlled and sustained release of multiple cardiovascular drugs

Light-responsive nanocomposite sponges for on demand chemical release with high spatial and dosage control

Rationalization of the entrapping of bioactive molecules into a series of functionalized porous zirconium terephthalate MOFs

Multifunctional hybrid silica nanoparticles for controlled doxorubicin loading and release with thermal and pH dual response

Protein recognition on a single graphene oxide surface fixed on a solid support

Disulfide-phenylazide: a reductively cleavable photoreactive linker for facile modification of nanoparticle surfaces

Photothermally enhanced photodynamic therapy based on mesoporous Pd@Ag@mSiO₂ nanocarriers

Magnetic nanoclusters with hydrophilic spacing for dual drug delivery and sensitive magnetic resonance imaging

Self-assembly of modified rhodamine-6G with tri-block copolymer: unusual vesicle formation, pH sensing and dye release properties

Highly stable quantum dots with silica–poly(EGDMA-co-MAA) synergistic protection and the preliminary application in immunoassay

Controlling performance of lipase immobilised on bioinspired silica

Mechanical strength and intracellular uptake of CaCO₃-templated LbL capsules composed of biodegradable polyelectrolytes: the influence of the number of layers

Molecular interactions between charged macromolecules: colorimetric detection and quantification of heparin with a polydiacetylene liposome

On the incorporation of functionalities into hydroxyapatite capsules

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Journal of Materials Chemistry B Vol - 1 Issue - 7

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Journal of Materials Chemistry B Vol - 1 Issue - 5

Controlling performance of lipase immobilised on bioinspired silica

Content Provider	Royal Society of Chemistry (RSC)
Author	Forsyth, Claire Patwardhan, Siddharth V.
Copyright Year	2013
Abstract	Lipase (Candida antarctica lipase B) was immobilised on silica that was produced via a mild route using a recently developed biologically inspired silica formation method. This route of immobilisation was favourable compared to traditional methods due to its simplicity, mild conditions, low cost, one-step procedure and short preparation time. Lipase was chosen as the enzyme due to its wide implementation in industry, particularly in the food and pharmaceutical industries. Compared to other methods using bioinspired silica entrapment of enzymes, we demonstrate several significant improvements in the performance of the biocatalysts produced. Very high immobilisation efficiencies (close to 100%) were achieved under optimised conditions. The immobilised enzymes also displayed high levels of activity, which exceeded those reported in previous studies of lipase that was immobilised on bioinspired silica. Furthermore, we demonstrate the control over enzyme activity which was achieved through the entrapment conditions by regulating surface area, the average pore diameter and the mean particle size of the silica support. The immobilised enzymes also had very good reuse potential, and showed improved thermal and pH stability. The biocatalysts also performed well in turbulent conditions, showed good storage properties and were successfully used in bench-top reactors, therefore strongly supporting their use in commercial applications. The immobilised enzymes also performed comparably to or better than the industrial benchmark, Novozym® 435. The bioinspired method of immobilisation used has significant benefits over current methods, and these factors could increase potential applications in industry.
Starting Page	1164
Ending Page	1174
Page Count	11
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	1
Issue Number	8
Journal	Journal of Materials Chemistry B
DOI	10.1039/c2tb00462c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Candida Lipase Candida albicans Silicon dioxide Silica Formation Enzyme PH
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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Formation of lipase Candida sp. 99–125 CLEAs in mesoporous silica: characterization and catalytic properties

Immobilising proteins on silica with site-specifically attached modified silaffin peptides

Metal-binding affinity of a series of bis-benzimidazoles immobilised on silica

A DNA-based strategy for dynamic positional enzyme immobilization inside fused silica microchannels

Facile one-pot synthesis of silica-based lipase nanocatalysts for improving stability

Facile immobilization of enzyme on three dimensionally ordered macroporous silica via a biomimetic coating

A drug delivery system based on switchable photo-controlled p-coumaric acid derivatives anchored on mesoporous silica

Synthesis of novel polyurethane polyesters using the enzyme Candida antarctica lipase B

Encapsulation of enzymes in silica nanocapsules formed by an amphiphilic precursor polymer in water

Controlling performance of lipase immobilised on bioinspired silica

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Metal-binding affinity of a series of bis-benzimidazoles immobilised on silica

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