NDLI: Aminofunctionalization and sub-micrometer patterning on silicon through silane doped agarose hydrogels

Content Provider	Royal Society of Chemistry (RSC)
Author	Marletta, Giovanni Silván, Miguel Manso Satriano, Cristina Messina, Grazia Maria Lucia Ruiz, Josefa Predestinación García Montero, Isabel
Copyright Year	2009
Abstract	Agarose based hydrogels doped with amino-propyl-triethoxy-silane have been prepared and used for the biofunctionalization of hydroxylated Si (100) surfaces. The doped hydrogels consist of two independent polysaccharide and siloxane networks. The confluent colloidal layer coverage of the substrate from the transferring hydrogels is illustrated by various techniques. The line shape of the N 1s and C 1s X-ray photoemission spectra is shown to depend strongly on the polar angle of the photoelectron emission. This effect is explained in terms of different indepth distributions of N and C species and the dominant presence of surface oriented amino groups. The surface modification induces an alteration of wettability, but the intrinsic nanoscale topography of the transferred films is the determinant factor of the interaction with water. Furthermore, it is found that the transferred functionalization is compatible with the formation of sub-micrometer patterns by exploiting as masks polystyrene based colloidal monolayers. Both nanodots and nanoporous wells with a hexagonal lattice were observed by atomic force microscopy and their biofunctionality demonstrated by fluorescence microscopy after fluorophore labelled IgG immobilization.
Starting Page	5226
Ending Page	5233
Page Count	8
File Format	HTM / HTML PDF
ISSN	09599428
Volume Number	19
Issue Number	29
Journal	Journal of Materials Chemistry
DOI	10.1039/b903820e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Polysaccharide Siloxane X-ray crystallography Photoemission spectroscopy Wetting Determinant Polystyrene Crystal structure Atomic-force microscopy Microscopy Fluorophore Immunoglobulin G
Content Type	Text
Resource Type	Article
Subject	Chemistry Materials Chemistry

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