NDLI: Detection and quantification of major toxigenic <i>Microcystis</i> genotypes in Moo-Tan reservoir and associated water treatment plant

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Detection and quantification of major toxigenic Microcystis genotypes in Moo-Tan reservoir and associated water treatment plant

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Back cover

Journal of Environmental Monitoring Vol -9

Journal of Environmental Monitoring Vol -8

Journal of Environmental Monitoring Vol -7

Journal of Environmental Monitoring Vol -6

Journal of Environmental Monitoring Vol -5

Journal of Environmental Monitoring Vol -4

Journal of Environmental Monitoring Vol -3

Journal of Environmental Monitoring Vol -2

Detection and quantification of major toxigenic Microcystis genotypes in Moo-Tan reservoir and associated water treatment plant

Content Provider	Royal Society of Chemistry (RSC)
Author	Tseng, I-Cheng Lin, Tsair-Fuh Yen, Hung-Kai
Copyright Year	2012
Abstract	Two molecular methods, denaturing gradient gel electrophoresis (DGGE) and quantitative real-time polymerase chain reaction (qPCR) with the Universal ProbeLibrary (UPL) probe, were developed and used for the characterization and quantification of several microcystin producers in Moo-Tan Reservoir (MTR), Taiwan and its associated water treatment plant (Shih-Men Water Treatment Plant, SMWTP). Internal transcribed spacer (ITS) sequence, a highly diversified region between the 16S rRNA and 23S rRNA genes, was used to further identify the isolated strains from MTR and also used in DGGE for the detection of the specific DNA fragments and biomarkers for 11 strains observed in MTR. These ITS-DGGE biomarkers were successfully applied to monitor the community changes of potential toxigenic Microcystis sp. over a period of five years. Two highly specific primers were combined with UPL probes to measure microcystins synthesis gene (mcyB) and phycocyanin intergenic spacer region (cpcB) concentrations in water samples. The copy concentrations of UPL-mcyB and UPL-cpcB correlated well with MC-RR concentrations/water temperature and Microcystis sp. cell numbers in the water samples, respectively. For SMWTP, toxin concentrations were low, but the DGGE bands clearly demonstrated the presence of potential microcystin producers in both water treatment plants and finished water samples. It was demonstrated that toxigenic Microcystis sp. may penetrate through the treatment processes and pose a potential risk to human health in the drinking water systems.
Starting Page	687
Ending Page	696
Page Count	10
File Format	HTM / HTML PDF
ISSN	14640325
Volume Number	14
Issue Number	2
Journal	Journal of Environmental Monitoring
DOI	10.1039/c1em10389j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Tan Reservoir Taiwan Gel electrophoresis Temperature gradient gel electrophoresis Polymerase Chain reaction Microcystin Methionine synthase Internal transcribed spacer 16S ribosomal RNA Ribosomal RNA DNA Microcystis Gene Phycocyanin Spacer DNA
Content Type	Text
Resource Type	Article
Subject	Public Health, Environmental and Occupational Health Management, Monitoring, Policy and Law

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Detection and quantification of major toxigenic Microcystis genotypes in Moo-Tan reservoir and associated water treatment plant

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