Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Hu, Shenghong Peng, Lu Zhu, Zhenli Guo, Wei Jin, Lanlan He, Qian |
| Copyright Year | 2014 |
| Abstract | A sensitive assay for detection of HIV-1 p24 antigen by inductively coupled plasma mass spectrometry (ICP-MS) was developed using a biotin–streptavidin (BA) system and gold nanoparticles (Au NPs) based immunoassay. In this immunoassay, the p24 antigen was firstly captured by an immobilized anti-HIV-1 p24 monoclonal antibody. After immunoreactions with the biotinylated anti-p24 polyclonal antibody and Au NPs-labeled streptavidin, diluted HNO3 (5%, v/v) was used to dissociate Au NPs, which were then introduced to the ICP-MS for measurements. Under the optimized conditions, the calibration graph for the p24 antigen was linear in the range of 7.5–75 pg mL−1 with a detection limit of 1.49 pg mL−1 (3σ, n = 5). The relative standard deviation (RSD) for three replicate measurements of 37.5 pg mL−1 of the p24 antigen was 3.7%. Other proteins, such as human IgG, human HSA, human CEA and human AFP, did not obviously interfere with the assay for p24 antigen. This method was also applied to measure p24 concentrations in artificially positive human serum samples. Compared with the biotin–streptavidin enzyme-linked immunosorbent assay (BA-ELISA) method for p24 antigen detection, the ICP-MS linked immunoassay process deals with Au NPs – tagged instead of enzyme-conjugated antibodies, making it free of toxic enzyme substrate reagents. In addition, it also simplifies the experimental process and saves the experimental time, since the color rendering steps are omitted. The proposed approach provides a sensitive method for HIV-1 p24 antigen determination. |
| Starting Page | 1477 |
| Ending Page | 1482 |
| Page Count | 6 |
| File Format | HTM / HTML PDF |
| ISSN | 02679477 |
| Volume Number | 29 |
| Issue Number | 8 |
| Journal | Journal of Analytical Atomic Spectrometry |
| DOI | 10.1039/c4ja00026a |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | HSA CEA AFP Subtypes of HIV Antigen Inductively coupled plasma mass spectrometry Immunoassay Monoclonal antibody Polyclonal antibodies Streptavidin Nitric acid Graph of a function Coefficient of variation Immunoglobulin G Alpha-fetoprotein ELISA Antibody Enzyme |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Analytical Chemistry |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|