NDLI: Three-dimensional hierarchical cultivation of human skin cells on bio-adaptive hybrid fibers

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Three-dimensional hierarchical cultivation of human skin cells on bio-adaptive hybrid fibers

Content Provider	Royal Society of Chemistry (RSC)
Author	Vukosavljevic, Branko Planz, Viktoria Seif, Salem Kroner, Elmar Sparenberg, Lisa Atchison, Jennifer S. Windbergs, Maike
Copyright Year	2016
Abstract	The human skin comprises a complex multi-scale layered structure with hierarchical organization of different cells within the extracellular matrix (ECM). This supportive fiber-reinforced structure provides a dynamically changing microenvironment with specific topographical, mechanical and biochemical cell recognition sites to facilitate cell attachment and proliferation. Current advances in developing artificial matrices for cultivation of human cells concentrate on surface functionalizing of biocompatible materials with different biomolecules like growth factors to enhance cell attachment. However, an often neglected aspect for efficient modulation of cell–matrix interactions is posed by the mechanical characteristics of such artificial matrices. To address this issue, we fabricated biocompatible hybrid fibers simulating the complex biomechanical characteristics of native ECM in human skin. Subsequently, we analyzed interactions of such fibers with human skin cells focusing on the identification of key fiber characteristics for optimized cell–matrix interactions. We successfully identified the mediating effect of bio-adaptive elasto-plastic stiffness paired with hydrophilic surface properties as key factors for cell attachment and proliferation, thus elucidating the synergistic role of these parameters to induce cellular responses. Co-cultivation of fibroblasts and keratinocytes on such fiber mats representing the specific cells in dermis and epidermis resulted in a hierarchical organization of dermal and epidermal tissue layers. In addition, terminal differentiation of keratinocytes at the air interface was observed. These findings provide valuable new insights into cell behaviour in three-dimensional structures and cell–material interactions which can be used for rational development of bio-inspired functional materials for advanced biomedical applications.
Starting Page	775
Ending Page	784
Page Count	10
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	8
Issue Number	7
Journal	Integrative Biology
DOI	10.1039/c6ib00080k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Extracellular matrix Biomaterial Stiffness Hydrophile Dermis Air interface
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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