NDLI: Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells

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Integrative Biology Vol -9

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Nanomedicine boosts neurogenesis: new strategies for brain repair

Giant vesicles as cell models

The impact of curcumin on breast cancer

Control of stem cell fate and function by engineering physical microenvironments

Nanopatterned cardiac cell patches promote stem cell niche formation and myocardial regeneration

Spatial confinement instigates environmental determination of neuronal polarity

Cancer develops, progresses and responds to therapies through restricted perturbation of the protein–protein interaction network

Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells

The study of polarisation in single cells using model cell membranes

The use of spectroscopic imaging and mapping techniques in the characterisation and study of DLD-1 cell spheroid tumour models

Nascent vessel elongation rate is inversely related to diameter in in vitro angiogenesis

Co-cultured endometrial stromal cells and peritoneal mesothelial cells for an in vitro model of endometriosis

The severity of mental disorders is linked to interaction among candidate genes

Fluid flow stress induced contraction and re-spread of mesenchymal stem cells: a microfluidic study

Plasma surface modification of electrospun fibers for adhesion-based cancer cell sorting

Tolfenamic acid suppresses cytochrome P450 2E1 expression in mouse liver

Biological network modeling identifies IPCS in Leishmania as a therapeutic target

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Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Schaffer, David V. Kumar, Sanjay Asuri, Prashanth Keung, Albert J.
Copyright Year	2012
Abstract	Human pluripotent stem cells (hPSCs) are of great interest in biology and medicine due to their ability to self-renew and differentiate into any adult or fetal cell type. Important efforts have identified biochemical factors, signaling pathways, and transcriptional networks that regulate hPSC biology. However, recent work investigating the effect of biophysical cues on mammalian cells and adult stem cells suggests that the mechanical properties of the microenvironment, such as stiffness, may also regulate hPSC behavior. While several studies have explored this mechanoregulation in mouse embryonic stem cells (mESCs), it has been challenging to extrapolate these findings and thereby explore their biomedical implications in hPSCs. For example, it remains unclear whether hPSCs can be driven down a given tissue lineage by providing tissue-mimetic stiffness cues. Here we address this open question by investigating the regulation of hPSC neurogenesis by microenvironmental stiffness. We find that increasing extracellular matrix (ECM) stiffness in vitro increases hPSC cell and colony spread area but does not alter self-renewal, in contrast to past studies with mESCs. However, softer ECMs with stiffnesses similar to that of neural tissue promote the generation of early neural ectoderm. This mechanosensitive increase in neural ectoderm requires only a short 5-day soft stiffness “pulse”, which translates into downstream increases in both total neurons as well as therapeutically relevant dopaminergic neurons. These findings further highlight important differences between mESCs and hPSCs and have implications for both the design of future biomaterials as well as our understanding of early embryonic development.
Starting Page	1049
Ending Page	1058
Page Count	10
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	4
Issue Number	9
Journal	Integrative Biology
DOI	10.1039/c2ib20083j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Cell potency Molecular biology Stiffness Laboratory mouse Neurogenesis Extracellular matrix Ant colony Germ layer Dopaminergic Biomaterial
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells

Soft Substrates Promote Homogeneous Self-Renewal of Embryonic Stem Cells via Downregulating Cell-Matrix (2010)

Rigid microenvironments promote cardiac differentiation of mouse and human embryonic stem cells

Combinatorial Extracellular Matrix Microenvironments for Probing Endothelial Differentiation of Human Pluripotent Stem Cells

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Combinatorial extracellular matrix microenvironments promote survival and phenotype of human induced pluripotent stem cell-derived endothelial cells in hypoxia

Tissue of Origin, but Not XCI State, Influences Germ Cell Differentiation from Human Pluripotent Stem Cells

Differentiation of Neural Progenitor Cells from Pluripotent Stem Cells in Artificial Niches

Lessons from the Embryonic Neural Stem Cell Niche for Neural Lineage Differentiation of Pluripotent Stem Cells

Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells

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Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells

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Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells