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Protein expression patterns of the yeast mating response

Gradually softening hydrogels for modeling hepatic stellate cell behavior during fibrosis regression

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Protein expression patterns of the yeast mating response

Content Provider	Royal Society of Chemistry (RSC)
Author	Zhang, Rongfei Ouyang, Qi Luo, Chunxiong Hao, Nan Wang, Xuan Yuan, Haiyu Shao, Bin
Copyright Year	2016
Abstract	Microfluidics, in combination with time-lapse microscopy, is a transformative technology that significantly enhances our ability to monitor and probe biological processes in living cells. However, high-throughput microfluidic devices mostly require sophisticated preparatory and setup work and are thus hard to adopt by non-experts. In this work, we designed an easy-to-use microfluidic chip, which enables tracking of 48 GFP-tagged yeast strains, with each strain under two different stimulus conditions, in a single experiment. We used this technology to investigate the dynamic pattern of protein expression during the yeast mating differentiation response. High doses of pheromone induce cell cycle arrest and the shmoo morphology, whereas low doses of pheromone lead to elongation and chemotrophic growth. By systematically analyzing the protein dynamics of 156 pheromone-regulated genes, we identified groups of genes that are preferentially induced in response to low-dose pheromone (elongation during growth) or high-dose pheromone (shmoo formation and cell cycle arrest). The protein dynamics of these genes may provide insights into the mechanisms underlying the differentiation switch induced by different doses of pheromone.
Starting Page	712
Ending Page	719
Page Count	8
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	8
Issue Number	6
Journal	Integrative Biology
DOI	10.1039/c6ib00014b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Microfluidics Time-lapse microscopy Yeast Protein Pheromone Cell cycle Shmoo Protein dynamics
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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Microfluidics-integrated time-lapse imaging for analysis of cellular dynamics

Microtubule Dynamics from Mating through the First Zygotic Division in the Budding Yeast Saccharomyces cerevisiae

Mitogen-activated protein kinase (MAPK) dynamics determine cell fate in the yeast mating response

Mating Pheromone in Cryptococcus neoformans Is Regulated by a Transcriptional/Degradative “Futile” Cycle

Cell cycle synchronization by nutrient modulation

Pheromone-encoded mRNA transport in mating yeast

The SPA2 gene of Saccharomyces cerevisiae is important for pheromone-induced morphogenesis and efficient mating.

Ceramide signals for initiation of yeast mating-specific cell cycle arrest

The SPA2 gene of Saccharomyces cerevisiae is important for pheromone-induced morphogenesis and efficient mating (1990)

Protein expression patterns of the yeast mating response

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Mitogen-activated protein kinase (MAPK) dynamics determine cell fate in the yeast mating response

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Protein expression patterns of the yeast mating response