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Microtubule Dynamics from Mating through the First Zygotic Division in the Budding Yeast Saccharomyces cerevisiae
| Content Provider | CiteSeerX |
|---|---|
| Abstract | Abstract. We have used time-lapse digital imaging microscopy to examine cytoplasmic astral microtubules (Mts) and spindle dynamics during the mating pathway in budding yeast Saccharomyces cerevisiae. Mating begins when two cells of opposite mating type come into proximity. The cells arrest in the G1 phase of the cell cycle and grow a projection towards one another forming a shmoo projection. Imaging of microtubule dynamics with green fluorescent protein (GFP) fusions to dynein or tubulin revealed that the nucleus and spindle pole body (SPB) became oriented and tethered to the shmoo tip by a Mt-dependent search and capture mechanism. Dynamically unstable astral Mts were captured at the shmoo tip forming a bundle of three or four astral Mts. This bundle changed length as the tethered nucleus |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Tethered Nucleus Astral Mt Shmoo Projection Green Fluorescent Protein Opposite Mating Type Spindle Dynamic Budding Yeast Saccharomyces Cerevisiae Yeast Saccharomyces Capture Mechanism Cytoplasmic Astral Microtubule Time-lapse Digital Imaging Microscopy G1 Phase Dynamically Unstable Astral Mt Shmoo Tip Cell Cycle Microtubule Dynamic First Zygotic Division Mt-dependent Search Spindle Pole Body |
| Content Type | Text |
| Resource Type | Article |
