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Next generation tools to accelerate the synthetic biology process

Computational fluid dynamics in the microcirculation and microfluidics: what role can the lattice Boltzmann method play?

Leveraging a high resolution microfluidic assay reveals insights into pathogenic fungal spore germination

Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry

Dynamic behaviors of astrocytes in chemically modified fibrin and collagen hydrogels

Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy

Monitoring protein synthesis in single live cancer cells

Deformability-based cell selection with downstream immunofluorescence analysis

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Dynamic behaviors of astrocytes in chemically modified fibrin and collagen hydrogels

Content Provider	Royal Society of Chemistry (RSC)
Author	Li, Yongchao Seyedhassantehrani, Negar Yao, Li
Copyright Year	2016
Abstract	Astrocytes play a critical role in supporting the normal physiological function of neurons in the central nervous system (CNS). Astrocyte transplantation can potentially promote axonal regeneration and functional recovery after spinal cord injury (SCI). Fibrin and collagen hydrogels provide growth-permissive substrates and serve as carriers for therapeutic cell transplantation into an injured spinal cord. However, the application of fibrin and collagen hydrogels may be limited due to their relatively rapid degradation rate in vivo. In this study, immature astrocytes isolated from neonatal rats were grown in fibrin hydrogels containing aprotinin and collagen hydrogels crosslinked with poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-StarPEG), and the cell behavior in these hydrogels was studied. The cell viability of astrocytes in the hydrogels was tested using the LIVE/DEAD® assay and the AlamarBlue® assay, and this study showed that astrocytes maintained good viability in these hydrogels. The cell migration study showed that astrocytes migrated in the fibrin and collagen hydrogels, and the migration speed is similar in these hydrogels. The crosslinking of collagen hydrogels with 4S-StarPEG did not change the astrocyte migration speed. However, the addition of aprotinin in the fibrin hydrogel inhibited astrocyte migration. The expression of chondroitin sulfate proteoglycan (CSPG), including NG2, neurocan, and versican, by astrocytes grown in the hydrogels was analyzed by quantitative RT-PCR. The expression of NG2, neurocan, and versican by the cells in these hydrogels was not significantly different.
Starting Page	624
Ending Page	634
Page Count	11
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	8
Issue Number	5
Journal	Integrative Biology
DOI	10.1039/c6ib00003g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Critical Role Central nervous system Astrocyte Spinal cord injury Fibrin Collagen Spinal cord Aprotinin Ethylene glycol Ether Glutaric acid The Cell Death Gel Chondroitin sulfate proteoglycan CSPG4 Neurocan Versican
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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NG2 is a major chondroitin sulfate proteoglycan produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors.

Entorhinal cortex lesion in adult rats induces the expression of the neuronal chondroitin sulfate proteoglycan neurocan in reactive astrocytes.

M1-type microglia can induce astrocytes to deposit chondroitin sulfate proteoglycan after spinal cord injury.

Neurocan: a brain chondroitin sulfate proteoglycan

Entorhinal cortex lesion in adult rats induces the expression of the neuronal chondroitin sulfate proteoglycan neurocan in reactive astrocytes.

Immunological identification of two proteoglycan fragments derived from neurocan, a brain-specific chondroitin sulfate proteoglycan

Chondroitin Sulfate Proteoglycan Immunoreactivity Increases Following Spinal Cord Injury and Transplantation

Statins decrease chondroitin sulfate proteoglycan expression and acute astrocyte activation in central nervous system injury

Role of the Chondroitin Sulfate Proteoglycan, Neurocan, in Inhibition of Sensory Neurite Regeneration

Dynamic behaviors of astrocytes in chemically modified fibrin and collagen hydrogels

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NG2 is a major chondroitin sulfate proteoglycan produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors.

Entorhinal cortex lesion in adult rats induces the expression of the neuronal chondroitin sulfate proteoglycan neurocan in reactive astrocytes.

M1-type microglia can induce astrocytes to deposit chondroitin sulfate proteoglycan after spinal cord injury.

Neurocan: a brain chondroitin sulfate proteoglycan

Entorhinal cortex lesion in adult rats induces the expression of the neuronal chondroitin sulfate proteoglycan neurocan in reactive astrocytes.

Immunological identification of two proteoglycan fragments derived from neurocan, a brain-specific chondroitin sulfate proteoglycan

Chondroitin Sulfate Proteoglycan Immunoreactivity Increases Following Spinal Cord Injury and Transplantation

Statins decrease chondroitin sulfate proteoglycan expression and acute astrocyte activation in central nervous system injury

Role of the Chondroitin Sulfate Proteoglycan, Neurocan, in Inhibition of Sensory Neurite Regeneration

Dynamic behaviors of astrocytes in chemically modified fibrin and collagen hydrogels