NDLI: Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes

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Contents list

The Discovery Channel: microfluidics and microengineered systems in drug screening

Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes

An in vitro model of tissue boundary formation for dissecting the contribution of different boundary forming mechanisms

Position along the nasal/temporal plane affects synaptic development by adult photoreceptors, revealed by micropatterning

PTEN deletion potentiates invasion of colorectal cancer spheroidal cells through 3D Matrigel

Directional migration and differentiation of neural stem cells within three-dimensional microenvironments

The direction of migration of T-lymphocytes under flow depends upon which adhesion receptors are engaged

Quantitating membrane bleb stiffness using AFM force spectroscopy and an optical sideview setup

Control of the interface between heterotypic cell populations reveals the mechanism of intercellular transfer of signaling proteins

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Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes

Content Provider	Royal Society of Chemistry (RSC)
Author	Qin, Jianhua Shi, Yang Zhang, Xu Li, Hongjing Jiang, Lei Ma, Jingyun
Copyright Year	2015
Abstract	Controlling the chondrocyte phenotype and function in a physiologically relevant microenvironment remains a major challenge for cartilage repair in tissue engineering applications. This work presents a straightforward strategy to create a high throughput concave microwell array used for generating multicellular spheroids of chondrocytes and facilitating the maintenance of the articular chondrocyte phenotype and function by combining 3D spheroid culture with hypoxia. The polydimethylsiloxane (PDMS) concave microwells were simply produced from a concave SU-8 template fabricated using a soft-lithography approach and easily adopted for size-controlled spheroid culture. 3D spheroid culture was observed to facilitate the cartilage-specific phenotype and function maintenance as compared to 2D monolayer culture. Combining hypoxia with spheroid culture markedly increased the expressions of cartilage-specific collagen II and aggrecan at protein and mRNA levels. The hypoxia-inducible factor (HIF) signaling pathway was found to get involved in phenotype maintenance, metabolism and differentiation of chondrocytes by regulating HIF-1α and HIF-2α, respectively. The established approach provides a useful platform for a wide range of applications in the field of cartilage biology, stem cell research and high throughput 3D drug testing in cancer.
Starting Page	289
Ending Page	297
Page Count	9
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	7
Issue Number	3
Journal	Integrative Biology
DOI	10.1039/c4ib00273c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Chondrocyte Phenotype Hyaline cartilage Tissue engineering Three-dimensional space Spheroid Tumor hypoxia Polydimethylsiloxane SU-8 photoresist Collagen Aggrecan Protein Messenger RNA Hypoxia-inducible factors Metabolism Stem cell Cancer
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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Regeneration of Hyaline Cartilage Using a Mechanically-Tuned Chondrocyte-Seeded Biomimetic Tissue-Engineered 3D Scaffold: A Theoretical Approach

Three-dimensional collagen scaffold.

BMP-2, hypoxia, and COL1A1/HtrA1 siRNAs favor neo-cartilage hyaline matrix formation in chondrocytes.

Enhanced hyaline cartilage matrix synthesis in collagen sponge scaffolds by using siRNA to stabilize chondrocytes phenotype cultured with bone morphogenetic protein-2 under hypoxia.

Direct Perfusion Improves Redifferentiation of Human Chondrocytes in Fibrin Hydrogel with the Deposition of Cartilage Pericellular Matrix

Induction and prevention of chondrocyte hypertrophy in culture (1989)

Fully Dedifferentiated Chondrocytes Expanded in Specific Mesenchymal Stem Cell Growth Medium with FGF2 Obtains Mesenchymal Stem Cell Phenotype In Vitro but Retains Chondrocyte Phenotype In Vivo

Chondrocytes, Mesenchymal Stem Cells, and Their Combination in Articular Cartilage Regenerative Medicine

Collagen Type II Articular Cartilage Pericellular Matrix DEVELOPMENT OF HYALINE-LIKE CARTILAGE BY PORCINE CHONDROCYTES IN PELLET CULTURE

Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes

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Regeneration of Hyaline Cartilage Using a Mechanically-Tuned Chondrocyte-Seeded Biomimetic Tissue-Engineered 3D Scaffold: A Theoretical Approach

Three-dimensional collagen scaffold.

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Enhanced hyaline cartilage matrix synthesis in collagen sponge scaffolds by using siRNA to stabilize chondrocytes phenotype cultured with bone morphogenetic protein-2 under hypoxia.

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Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes