NDLI: Pump-free multi-well-based microfluidic system for high-throughput analysis of size-control relative genes in budding yeast

Content Provider	Royal Society of Chemistry (RSC)
Author	Jiang, Lingli Chen, Xi Ouyang, Qi Luo, Chunxiong Yuan, Haiyu Kang, Xianjie
Copyright Year	2014
Abstract	Time-lapse single cell imaging by microscopy can provide precise cell information such as the cell size, the cell cycle duration, protein localization and protein expression level. Usually, a microfluidic system is needed for these measurements in order to provide a constant culture environment and confine the cells so that they grow in a monolayer. However, complex connections are required between the channels inside the chip and the outside media, and a complex procedure is needed for loading of cells, thereby making this type of system unsuitable for application in high-throughput single cell scanning experiments. Here we provide a novel and easily operated pump-free multi-well-based microfluidic system which enables the high-throughput loading of many different budding yeast strains into monolayer growth conditions just by use of a multi-channel pipette. Wild type budding yeast (Saccharomyces cerevisiae) and 62 different budding yeast size control relative gene deletion strains were chosen for scanning. We obtained normalized statistical results for the mother cell doubling time, daughter cell doubling time, mother cell size and daughter cell size of different gene deletion strains relative to the corresponding parameters of the wild type cells. Meanwhile, we compared the typical cell morphology of different strains and analyzed the relationship between the cell genotype and phenotype. This method which can be easily used in a normal biology lab may help researchers who need to carry out the high-throughput scanning of cell morphology and growth.
Starting Page	685
Ending Page	693
Page Count	9
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	6
Issue Number	7
Journal	Integrative Biology
DOI	10.1039/c4ib00054d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Time-lapse photography Microscopy Cell cycle Protein Pipette Wild type Saccharomyces cerevisiae Gene Genotype Phenotype Molecular biology
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae (1995)

Microtubule Dynamics from Mating through the First Zygotic Division in the Budding Yeast Saccharomyces cerevisiae

Mathematical model of the budding yeast cell cycle (2000).

Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.

The GTS1 gene, which contains a Gly-Thr repeat, affects the timing of budding and cell size of the yeast Saccharomyces cerevisiae (1994)

Characterization of the saccharomyces cerevisiae fol1 protein: starvation for c1 carrier induces pseudohyphal growth (2003).

Life cycle of the budding yeast Saccharomyces cerevisiae.

Regulation of the saccharomyces cerevisiae whz2 gene (1989).

Cell cycle synchronization by nutrient modulation

Pump-free multi-well-based microfluidic system for high-throughput analysis of size-control relative genes in budding yeast

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Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae (1995)

Microtubule Dynamics from Mating through the First Zygotic Division in the Budding Yeast Saccharomyces cerevisiae

Mathematical model of the budding yeast cell cycle (2000).

Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.

The GTS1 gene, which contains a Gly-Thr repeat, affects the timing of budding and cell size of the yeast Saccharomyces cerevisiae (1994)

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Life cycle of the budding yeast Saccharomyces cerevisiae.

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Pump-free multi-well-based microfluidic system for high-throughput analysis of size-control relative genes in budding yeast