NDLI: Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology and proliferation of T47D breast carcinoma cells

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Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology and proliferation of T47D breast carcinoma cells

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Integrative Biology Vol - 5 Issue - 2

Integrative Biology Vol -4

Integrative Biology Vol -3

Integrative Biology Vol -2

Integrative Biology Vol -1

Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology and proliferation of T47D breast carcinoma cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Montanez-Sauri, Sara I. Beebe, David J. Sung, Kyung Eun Berthier, Erwin
Copyright Year	2013
Abstract	During breast carcinoma progression, the three-dimensional (3D) microenvironment is continuously remodeled, and changes in the composition of the extracellular matrix (ECM) occur. High throughput screening platforms have been used to decipher the complexity of the microenvironment and to identify ECM components responsible for cancer progression. However, traditional screening platforms are typically limited to two-dimensional (2D) cultures, and often exclude the influence of ECM and stromal components. In this work, a system that integrates 3-dimensional cell culture techniques with an automated microfluidic platform was used to create a new ECM screening platform that cultures cells in more physiologically relevant 3D in vitro microenvironments containing stromal cells and different ECM molecules. This new ECM screening platform was used to culture T47D breast carcinoma cells in mono- and co-culture with human mammary fibroblasts (HMF) with seven combinations of three different ECM proteins (collagen, fibronectin, laminin). Differences in the morphology of T47D clusters, and the proliferation of T47D cells were found in ECM compositions rich in fibronectin or laminin. In addition, an MMP enzyme activity inhibition screening showed the capabilities of the platform for small molecule screening. The platform presented in this work enables screening for the effects of matrix and stromal compositions and show promises for providing new insights in the identification of key ECM components involved in breast cancer.
Starting Page	631
Ending Page	640
Page Count	10
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	5
Issue Number	3
Journal	Integrative Biology
DOI	10.1039/c3ib20225a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	ECM MMP Carcinoma Three-dimensional space Extracellular matrix High-throughput screening Cancer Cell culture List of breast cancer cell lines Collagen Fibronectin Laminin Enzyme Breast cancer
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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Enabling Screening in 3D Microenvironments: Probing Matrix and Stromal Effects on the Morphology and Proliferation of T47D Breast Carcinoma Cells

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Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology and proliferation of T47D breast carcinoma cells

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