NDLI: Using fluorescent pH-sensitive nanosensors to report their intracellular location after Tat-mediated delivery

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Changing the face of scientific publishing

Direct measurement of Mycobacterium–fibronectin interactions

Pulling force generated by interacting SNAREs facilitates membrane hemifusion

Unbiased discovery of in vivo imaging probes through in vitro profiling of nanoparticle libraries

Using fluorescent pH-sensitive nanosensors to report their intracellular location after Tat-mediated delivery

The natural transparency and piezoelectric response of the Greta oto butterfly wing

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Using fluorescent pH-sensitive nanosensors to report their intracellular location after Tat-mediated delivery

Content Provider	Royal Society of Chemistry (RSC)
Author	Briddon, Stephen J. Aylott, Jonathan W. Coupland, Paul G.
Copyright Year	2009
Abstract	Nanosensors offer the opportunity to measure intracellular domains with minimal chemical or physical perturbation. Typically only 50 nm in diameter, and synthesised from bio-compatible polymer matrices, they entrap chemical sensing elements and can be surface functionalised allowing for further chemical modification. Upon intracellular localisation the cellular environment can be monitored using conventional techniques such as confocal laser scanning microscopy. Reported here is the use of nanosensors to investigate the mechanisms of intracellular delivery mediated via the cell penetrating peptide, Tat. Information obtained from the nanosensors shows that the post-delivery environment is representative of a lysosome in terms of both pH and morphology. The delivery mechanism of Tat, however, is shown to be dependent upon the cargo being delivered, corresponding to the absence or presence of a body of polymer matrix; thus nanosensors have been used to further the understanding of the cell penetrating mechanisms of Tat peptide.
Starting Page	318
Ending Page	323
Page Count	6
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	1
Issue Number	4
Journal	Integrative Biology
DOI	10.1039/b822569a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Nanosensors Tat Nanosensor Polymer Confocal microscopy Cell-penetrating peptide Structure and genome of HIV Lysosome PH Extracellular matrix Peptide
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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