NDLI: Plasma surface modification of electrospun fibers for adhesion-based cancer cell sorting

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Integrative Biology Vol -9

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Nanomedicine boosts neurogenesis: new strategies for brain repair

Giant vesicles as cell models

The impact of curcumin on breast cancer

Control of stem cell fate and function by engineering physical microenvironments

Nanopatterned cardiac cell patches promote stem cell niche formation and myocardial regeneration

Spatial confinement instigates environmental determination of neuronal polarity

Cancer develops, progresses and responds to therapies through restricted perturbation of the protein–protein interaction network

Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells

The study of polarisation in single cells using model cell membranes

The use of spectroscopic imaging and mapping techniques in the characterisation and study of DLD-1 cell spheroid tumour models

Nascent vessel elongation rate is inversely related to diameter in in vitro angiogenesis

Co-cultured endometrial stromal cells and peritoneal mesothelial cells for an in vitro model of endometriosis

The severity of mental disorders is linked to interaction among candidate genes

Fluid flow stress induced contraction and re-spread of mesenchymal stem cells: a microfluidic study

Plasma surface modification of electrospun fibers for adhesion-based cancer cell sorting

Tolfenamic acid suppresses cytochrome P450 2E1 expression in mouse liver

Biological network modeling identifies IPCS in Leishmania as a therapeutic target

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Plasma surface modification of electrospun fibers for adhesion-based cancer cell sorting

Content Provider	Royal Society of Chemistry (RSC)
Author	Blackstone, B. N. Nelson, M. T. Hart, R. T. Powell, H. M. Willard, J. J. Lee, C. H. Lannutti, J. J.
Copyright Year	2012
Abstract	Personalized cancer therapies drive the need for devices that rapidly and accurately segregate cancer cells from solid tumors. One potential sorting strategy is to segregate populations of cells based on their relative strength of adhesion. To investigate the effect of surface hydrophilicity and cell phenotype on adhesion, primary human breast skin fibroblasts and keratinocytes and MCF-7 breast cancer cells were seeded onto air and CF4 plasma-treated nanofibers followed by exposure to three shear stresses (200, 275 and 350 dynes per cm2) 1 hour after inoculation. No difference in strength of adhesion was measured in either fibroblasts or keratinocytes on either plasma treated-surface: all exhibited >60% of the initial cell count after a 5 minute exposure to 350 dynes per cm2 of shear stress. In contrast, a significant difference between relative strength of adhesion on air versus CF4 plasma-treated surfaces was observed for MCF-7 cells: 26% and 6.6% of cells remained on the air and CF4 plasma-treated surfaces, respectively. The ability to sort this cancer cell line from two non-cancerous primary human cells was evaluated by inoculating a mixture of all three cell types simultaneously onto CF4 treated nanofibers followed by 1 hour of culture and exposure to 350 dynes per cm2 shear stress. The majority of MCF-7 cells were removed (0.7% remained) while a majority of fibroblasts and keratinocytes remained adhered (74 and 57%). Post-sorted MCF-7 viability and morphology remained unchanged, preserving the possibility of post-separation and analysis. These data suggest that the plasma treatment of electrospun scaffolds provides a tool useful in sorting cancer cells from a mixed cell population based on adhesion strength.
Starting Page	1112
Ending Page	1121
Page Count	10
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	4
Issue Number	9
Journal	Integrative Biology
DOI	10.1039/c2ib20025b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Cancer Hydrophile Phenotype MCF-7 Breast cancer Inoculation Blood plasma Shear stress
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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1876-5319/10 2010 Bentham Open Open Access Effect of Tetrafluoromethane Plasma Treatment of PMMA on MCF-7 Cell Proliferation

Amphotericin B potentiates the anticancer activity of doxorubicin on the MCF-7 breast cancer cells.

Amphotericin B potentiates the anticancer activity of doxorubicin on the MCF-7 breast cancer cells

Retinoic acid increases tyrosine phosphorylation of focal adhesion kinase and paxillin in mcf-7 human breast cancer cells. Cancer res (1999).

Combination of a Novel Photosensitizer DTPP with 650 nm Laser Results in Efficient Apoptosis and Cytoskeleton Collapse in Breast Cancer MCF-7 Cells

Fluid shear stress modulates surface expression of adhesion molecules by endothelial cells

Changes in Electric Properties of Human Breast Cancer Cells

Human preadipocytes inhibit proliferation of mcf-7 breast cancer cell line.

Cadherin-23 Mediates Heterotypic Cell-Cell Adhesion between Breast Cancer Epithelial Cells and Fibroblasts (2011)

Plasma surface modification of electrospun fibers for adhesion-based cancer cell sorting

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Amphotericin B potentiates the anticancer activity of doxorubicin on the MCF-7 breast cancer cells

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Plasma surface modification of electrospun fibers for adhesion-based cancer cell sorting