NDLI: Non-invasive microfluidic gap junction assay

Please wait, while we are loading the content...

Microfluidic electroporation of tumor and blood cells: observation of nucleus expansion and implications on selective analysis and purging of circulating tumor cells

Synthesis of ternary CuInS₂/ZnS quantum dot bioconjugates and their applications for targeted cancer bioimaging

Non-invasive microfluidic gap junction assay

Dynamics of human cancer cell lines monitored by electrical and acoustic fluctuation analysis

Back cover

Integrative Biology Vol -1

Non-invasive microfluidic gap junction assay

Content Provider	Royal Society of Chemistry (RSC)
Author	Chen, Sisi Lee, Luke P.
Copyright Year	2010
Abstract	Gap junctions are protein channels between cells that allow direct electrical and metabolic coupling via the exchange of biomolecules and ions. Their expression, though ubiquitous in most mammalian cell types, is especially important for the proper functioning of cardiac and neuronal systems. Many existing methods for studying gap junction communication suffer from either unquantifiable data or difficulty of use. Here, we measure the extent of dye spread and effective diffusivities through gap junction connected cells using a quantitative microfluidic cell biology platform. After loading dye by hydrodynamic focusing of calcein/AM, dye transfer dynamics into neighboring, unexposed cells can be monitored via timelapse fluorescent microscopy. By using a selective microfluidic dye loading over a confluent layer of cells, we found that high expression of gap junctions in C6 cells transmits calcein across the monolayer with an effective diffusivity of 3.4 × 10−13 m2/s, which are highly coupled by Cx43. We also found that the gap junction blocker 18α-GA works poorly in the presence of serum even at high concentrations (50 µM); however, it is highly effective down to 2.5 µM in the absence of serum. Furthermore, when the drug is washed out, dye spread resumes rapidly within 1 min for all doses, indicating the drug does not affect transcriptional regulation of connexins in these Cx43+ cells, in contrast to previous studies. This integrated microfluidic platform enables the in situ monitoring of gap junction communication, yielding dynamic information about intercellular molecular transfer and pharmacological inhibition and recovery.
Starting Page	130
Ending Page	138
Page Count	9
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	2
Issue Number	2-3
Journal	Integrative Biology
DOI	10.1039/b919392h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Gap Inc. Protein Theoretical neuromorphology Gap junction Staining Molecular biology Calcein Amplitude modulation Microscopy Reuptake inhibitor
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Modulation of gap junction channels and hemichannels by growth factors

Connexins and the gap in context

Protection of Retinal Cells from Ischemia by a Novel Gap Junction Inhibitor

Identification of Cx45 as a Major Component of GJs in HeLa Cells

Oligomeric Structure and Functional Characterization of Caenorhabditis elegans Innexin-6 Gap Junction Protein

Gap junction channel gating modulated through protein phosphorylation

Impaired Gap Junction Formation and Intercellular Calcium Signaling in Urinary Bladder Cancer Cells can be Improved by Gö6976

Gap junction-ribosome association after autolysis.

Protection from Ataxia-linked Apoptosis by Gap Junction Inhibitors

Non-invasive microfluidic gap junction assay

Similar Documents

Modulation of gap junction channels and hemichannels by growth factors

Connexins and the gap in context

Protection of Retinal Cells from Ischemia by a Novel Gap Junction Inhibitor

Identification of Cx45 as a Major Component of GJs in HeLa Cells

Oligomeric Structure and Functional Characterization of Caenorhabditis elegans Innexin-6 Gap Junction Protein

Gap junction channel gating modulated through protein phosphorylation

Impaired Gap Junction Formation and Intercellular Calcium Signaling in Urinary Bladder Cancer Cells can be Improved by Gö6976

Gap junction-ribosome association after autolysis.

Protection from Ataxia-linked Apoptosis by Gap Junction Inhibitors

Non-invasive microfluidic gap junction assay