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Integrative Biology Vol -1

Applications of modern micro-Raman spectroscopy for cell analyses

Content Provider	Royal Society of Chemistry (RSC)
Author	Day, Philip J. R. Mariani, Melissa M. Deckert, Volker
Copyright Year	2010
Abstract	Raman spectroscopy assesses the chemical composition of a sample by exploiting the inherent and unique vibrational chacteristics of chemical bonds. Initial applications of Raman were identified in the industrial and chemical sectors, providing a rapid non-invasive method to identify sample components or perform quality control assessments. Applications have since increased and sample sizes decreased, leading to the onset of micro-Raman spectroscopy. Coupling with microscopy enabled label-free sample analysis and the unveiling of total chemical composition. Latter adaptations of Raman have advanced into biomedical diagnostics and research. Alongside technical developments in filter systems and detectors, spectral peak intensities and improved signal-to-noise ratios have facilitated target molecule measurement within a variety of samples. Quantitative sample analysis applications of Raman have contributed to its increasing popularity. Through these exceptional capabilites, potential Raman spectroscopy utility in biomedical research applications has expanded, exemplifying why there is continued interest in this highly sensitive and often under-used technique.
Starting Page	94
Ending Page	101
Page Count	8
File Format	HTM / HTML PDF
ISSN	17579694
Volume Number	2
Issue Number	2-3
Journal	Integrative Biology
DOI	10.1039/b920572a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Raman Raman spectroscopy Spectroscopy Microscopy Signal-to-noise ratio
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biophysics

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