NDLI: Anti-inflammatory effects of pomegranate (<i>Punica granatum</i> L.) husk ellagitannins in Caco-2 cells, an <i>in vitro</i> model of human intestine

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Vitamin D insufficiency and chronic diseases: Hype and reality

The health benefits of blackcurrants

Prenylated isoflavonoids from plants as selective estrogen receptor modulators (phytoSERMs)

Mango fruit peel and flesh extracts affect adipogenesis in 3T3-L1 cells

Protective role of Brassica olerecea and Eugenia jambolana extracts against H₂O₂ induced cytotoxicity in H9C2 cells

Hypericum androsaemum water extract inhibits proliferation in human colorectal cancer cells through effects on MAP kinases and PI3K/Akt pathway

The soluble dietary fiber inulin can influence the bioaccessibility of enniatins

Complexation of high methoxyl pectin with ethanol desolvated whey protein nanoparticles: physico-chemical properties and encapsulation behaviour

Hypotensive, hypoglycaemic and antioxidant effects of consuming a cocoa product in moderately hypercholesterolemic humans

Anti-inflammatory effects of pomegranate (Punica granatum L.) husk ellagitannins in Caco-2 cells, an in vitro model of human intestine

Scopoletin attenuates allergy by inhibiting Th2 cytokines production in EL-4 T cells

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Anti-inflammatory effects of pomegranate (Punica granatum L.) husk ellagitannins in Caco-2 cells, an in vitro model of human intestine

Content Provider	Royal Society of Chemistry (RSC)
Author	Hollebeeck, Sylvie Hérent, Marie-France During, Alexandrine Larondelle, Yvan Winand, Julie Leclercq, Joëlle Schneider, Yves-Jacques
Copyright Year	2012
Abstract	This study aimed at evaluating the anti-inflammatory properties of a pomegranate fruit husk (PomH) polyphenolic extract, rich in punicalagin, using Caco-2 cells, an in vitro model of human intestinal epithelium. Differentiated cells in bicameral inserts were pretreated or not with a PomH extract or punicalagin, as reference, at the apical side, representing the intestinal lumen. Inflammation was then induced with a cocktail of cytokines (Il-1β, TNFα and IFNγ) and LPS. After 24 h incubation, 3 pro-inflammatory markers, i.e., interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1, were assayed both at their gene transcription (qRT-PCR) and secretion (ELISA) levels. As previously described, the pro-inflammatory cocktail significantly stimulated these 3 markers, at the gene transcript and secretion levels. In inflamed cells, a significant down-regulation of the transcription of the genes encoding IL-6 and MCP-1 was observed in the presence of the PomH extract or punicalagin, while IL-8 transcription was unaffected. Both treatments also decreased the amounts of the 3 proteins with dose-response effects, but only in the apical compartment. A lowered ELISA response was also observed when either IL-6, IL-8 or MCP-1 were mixed with punicalagin in a cell-free culture medium, indicating a direct molecular interaction. In conclusion, the punicalagin-rich PomH extract tested showed anti-inflammatory properties in the Caco-2 in vitro intestinal model. It acted both on the pro-inflammatory gene transcription and protein levels, the later phenomenon being possibly due to a direct molecular trapping. These data suggest that pomegranate husk could be an interesting natural source contributing to prevent intestinal chronic inflammation.
Starting Page	875
Ending Page	885
Page Count	11
File Format	HTM / HTML PDF
ISSN	20426496
Volume Number	3
Issue Number	8
Journal	Food & Function
DOI	10.1039/c2fo10258g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	ELISA Pomegranate Punicalagin Caco-2 Epithelium Bicameralism Thylakoid Cocktail Interleukin 1 beta Tumor necrosis factor alpha Interferon gamma Honduran lempira Interleukin 8 Monocyte Chemotaxis Protein Gene Real-time polymerase chain reaction Interleukin 6 Inflammation
Content Type	Text
Resource Type	Article
Subject	Medicine Food Science

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Rapid large scale purification of ellagitannins from pomegranate husk, a by-product of the commercial juice industry

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Anti-inflammatory effects of pomegranate (Punica granatum L.) husk ellagitannins in Caco-2 cells, an in vitro model of human intestine

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