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Environmental Science: Processes & Impacts Vol - 18 Issue - 6

Environmental Science: Processes & Impacts Vol - 18 Issue - 5

Spectroscopic evaluation of the interaction between pesticides and chickpea cystatin: comparative binding and toxicity analyses

Content Provider	Royal Society of Chemistry (RSC)
Author	Bano, Bilqees Bhat, Sheraz Ahmad Bhat, Waseem Feeroze
Copyright Year	2016
Abstract	The binding study of pesticides with proteins is of great importance in ecotoxicology. In this study, a comparative interaction mechanism of phytocystatin with three pesticides has been presented, each from a different class-glyphosate herbicide (GPS), chlorpyrifos insecticide (CPF), and mancozeb fungicide (MCZ). The interaction of purified chickpea cystatin (CPC) has been characterized by fluorescence, UV, and circular dichroism (CD) spectroscopic methods. The study revealed association constants (Ka) of 52 M−1, 1.145 × 103 M−1, and 36.12 M−1 for the interaction of CPF, MCZ, and GPS with CPC, respectively, signifying the high affinity interaction for MCZ. Structural changes (at tertiary and secondary levels) were confirmed by UV-visible, intrinsic fluorescence and CD spectroscopy. The results showed that the effect on the CPC structure was more pronounced in the case of MCZ, which was followed by CPF and then GPS. The functional analysis of the pesticide treated inhibitor showed a decline in antipapain activity which varied with the time and dose as well as the class of pesticide. MCZ was relatively much more toxic as compared to CPF and GPS. Reactive oxygen species responsible for inhibitor damage were also analyzed. The results obtained implicate that the exposure of plants to pesticides may lead to physicochemical changes in proteins such as phytocystatins leading to physiological damage to the plant system.
Starting Page	872
Ending Page	881
Page Count	10
File Format	HTM / HTML PDF
ISSN	20507887
Volume Number	18
Issue Number	7
Journal	Environmental Science: Processes & Impacts
DOI	10.1039/c6em00195e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	CPC CPF MCZ Ecotoxicology Herbicide Global Positioning System Chlorpyrifos Insecticide Fungicide Chickpea Cystatin Fluorescence Circular dichroism Spectroscopy Functional analysis Pesticide Enzyme inhibitor Reactive oxygen species Plant System
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Public Health, Environmental and Occupational Health Management, Monitoring, Policy and Law

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