NDLI: Silver nanoparticle–protein interactions in intact rainbow trout gill cells

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Back cover

Environmental Science: Nano Vol - 3 Issue - 4

Environmental Science: Nano Vol - 3 Issue - 3

Environmental Science: Nano Vol - 3 Issue - 2

Environmental Science: Nano Vol - 3 Issue - 1

Environmental Science: Nano Vol -2

Environmental Science: Nano Vol -1

Silver nanoparticle–protein interactions in intact rainbow trout gill cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Suter, Marc J.-F. Sigg, Laura Yue, Yang Pillai, Smitha Behra, Renata Schirmer, Kristin
Copyright Year	2016
Abstract	Upon contact with biota, nanoparticles can bind to proteins, which coat the nanoparticles and form a nanoparticle-protein corona. Knowledge of corona proteins is therefore important for a mechanistic understanding of how nanoparticles interact with biomolecules in cells. Here we present the first study to reveal the identity of corona proteins from silver nanoparticle (AgNPs)-exposed living vertebrate cells. The cells are from a rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1, representing the interface between the aquatic environment and one of its model species. Subcellular fractionation allowed AgNP-protein corona complexes to be recovered from intact subcellular compartments and proteins lysed from the AgNPs to be detected by mass spectrometry. The identified proteins mark the trail of AgNPs processing in the cells like a forensic fingerprint: the cells take up the AgNPs via endocytic processes and store the particles in endosomal/lysosomal compartments. Moreover, stress response proteins were recovered in the AgNPs protein corona. In this way, we established a list of AgNPs susceptible proteins which can be investigated further in targeted nanoparticle–protein interaction. As a proof of principle, we demonstrate that Na+/K+-ATPase, identified from the corona and a known key protein in ion regulation in gill cells, is inhibited in its activity by AgNPs, confirming previously published in vivo experiments. The developed methodology is broadly applicable to other nanoparticles and cell types, representing a valuable tool for mechanistic nanoparticle–cell interaction studies, ranging from environmental and human risk assessment to biomedicine. In this way, our research also contributes to safer particle design.
Starting Page	1174
Ending Page	1185
Page Count	12
File Format	HTM / HTML PDF
ISSN	20518153
Volume Number	3
Issue Number	5
Journal	Environmental Science: Nano
DOI	10.1039/c6en00119j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Life Corona discharge Interaction Silver nanoparticle Vertebrate The Cells Rainbow trout Gill Mass spectrometry Trail Fingerprint Protein Ion Biomedicine
Content Type	Text
Resource Type	Article
Subject	Environmental Science Materials Science

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