NDLI: Rapid cell extraction in aqueous two-phase microdroplet systems

Content Provider	Royal Society of Chemistry (RSC)
Author	Vijayakumar, Kalpana deMello, Andrew J. Gulati, Shelly Edel, Joshua B.
Copyright Year	2010
Abstract	Distinguishing specific cells is an essential technique in cell research and clinical diagnostics. We report a novel method to passively isolate and extract cells in a microfluidic device. We utilise a droplet-based microfluidic system to generate an aqueous two phase system in which aqueous droplets consist of two phases in the form of a double emulsion. Specifically, we generate PEG droplets that completely encapsulate DEX droplets within a microfluidic channel. Target cells can be introduced directly into the droplets and driven to partition to the more favourable phase, whilst still being contained within the aqueous droplet. Human T lymphoma cells, with diameters in the range of 10–15 μm, are chosen as a model cell line to demonstrate the partitioning.
Starting Page	447
Ending Page	452
Page Count	6
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	1
Issue Number	4
Journal	Chemical Science
DOI	10.1039/c0sc00229a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Emulsion DEX Micrometre Lymphoma
Content Type	Text
Resource Type	Article
Subject	Chemistry

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Rapid cell extraction in aqueous two-phase microdroplet systems