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Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
| Content Provider | Scilit |
|---|---|
| Author | Yan, Xu Souza, Maria Aparecida Pontes, Marcela Z. R. Vitolo, Michele Júnior, Adalberto Pessoa |
| Copyright Year | 2003 |
| Description | From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented. |
| Related Links | http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1516-89132003000400030&lng=en&nrm=iso&tlng=en |
| Ending Page | 750 |
| Page Count | 10 |
| Starting Page | 741 |
| ISSN | 15168913 |
| e-ISSN | 16784324 |
| DOI | 10.1590/s1516-89132003000400030 |
| Journal | Brazilian Archives of Biology and Technology |
| Issue Number | 4 |
| Volume Number | 46 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2003-12-01 |
| Access Restriction | Open |
| Subject Keyword | Brazilian Archives of Biology and Technology Analytical Chemistry Liquid Liquid Extraction Affinity Aqueous Aqueous Two Phase Systems |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
