NDLI: Parallel folding topology-selective label-free detection and monitoring of conformational and topological changes of different G-quadruplex DNAs by emission spectral changes <i>via</i> FRET of <i>m</i>PPE-Ala

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Chemical Science Vol - 7 Issue - 3

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Parallel folding topology-selective label-free detection and monitoring of conformational and topological changes of different G-quadruplex DNAs by emission spectral changes via FRET of mPPE-Ala–Pt(ii) complex ensemble

Content Provider	Royal Society of Chemistry (RSC)
Author	Chan, Kevin Chung, Clive Yik-Sham Yam, Vivian Wing-Wah
Copyright Year	2016
Abstract	The formation of supramolecular assemblies between [Pt(bzimpy-Et){CCC6H4(CH2NMe3-4)}]Cl2 (1) and mPPE-Ala and the FRET properties of the ensemble have been revealed from the UV-vis absorption, steady-state emission and time-resolved emission decay studies. The two-component mPPE-Ala–1 ensemble has been employed in a “proof-of-principle” concept for label-free detection of G-quadruplex DNAs with the intramolecular propeller parallel folding topology, such as c-myc, in aqueous buffer solution. By the modulation of the aggregation/deaggregation of the polymer–metal complex aggregates and hence the FRET from the mPPE-Ala donor to the aggregated 1 as acceptor, the ensemble has been demonstrated for sensitive and selective label-free detection of c-myc via the monitoring of emission spectral changes of the ensemble. Ratiometric emission of the ensemble at 461 and 662 nm has been shown to distinguish the intramolecular propeller parallel G-quadruplex folding topology of c-myc from other G-quadruplex-forming sequences of different folding topologies, owing to the strong and specific interactions between c-myc and 1 as suggested by the UV-vis absorption and UV melting studies. In addition, the formation of high-order intermolecular multimeric G-quadruplexes from c-myc under molecular crowding conditions has been successfully probed by the ratiometric emission of the ensemble. The conformational and topological transition of human telomeric DNA from the mixed-hybrid form to the intramolecular propeller parallel form, as observed from the circular dichroism spectroscopy, has also been monitored by the ratiometric emission of the ensemble. The ability of the ensemble to detect these conformational and topological transitions of G-quadruplex DNAs has been rationalized by the excellent selectivity and sensitivity of the ensemble towards the intramolecular propeller parallel G-quadruplex DNAs and their high-order intermolecular multimers, which are due to the extra stabilization gained from Pt⋯Pt and π–π interactions in addition to the electrostatic and hydrophobic interactions found in the polymer–metal complex aggregates.
Starting Page	2842
Ending Page	2855
Page Count	14
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	7
Issue Number	4
Journal	Chemical Science
DOI	10.1039/c5sc04563k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Absorption spectroscopy Förster resonance energy transfer Myc DNA G-quadruplex Topology Hydrophobe Rings of Saturn Buffer solution Circular dichroism
Content Type	Text
Resource Type	Article
Subject	Chemistry

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Parallel folding topology-selective label-free detection and monitoring of conformational and topological changes of different G-quadruplex DNAs by emission spectral changes via FRET of mPPE-Ala–Pt(ii) complex ensemble

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