NDLI: Development of kartogenin-conjugated chitosan–hyaluronic acid hydrogel for nucleus pulposus regeneration

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Biomaterials Science Vol - 5 Issue - 3

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Biomaterials Science Vol - 5 Issue - 1

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Biomaterials Science Vol -3

Biomaterials Science Vol -2

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Development of kartogenin-conjugated chitosan–hyaluronic acid hydrogel for nucleus pulposus regeneration

Content Provider	Royal Society of Chemistry (RSC)
Author	Lin, Guangyao Wang, Yiwei Song, Kedong Zhou, Guangqian Zhu, Hongxia Wang, Liang Zhu, Yanxia Yi, Weihong Tan, Jie Yin, Fei
Copyright Year	2017
Abstract	Injectable constructs for in vivo gelation have many advantages in the regeneration of degenerated nucleus pulposus. In this study, an injectable hydrogel consisting of chitosan (CS) and hyaluronic acid (HA) crosslinked with glycerol phosphate (GP) at different proportions (CS : GP : HA, 6 : 3 : 1, 5 : 3 : 2, 4 : 3 : 3, 3 : 3 : 4, 2 : 3 : 5, 1 : 3 : 6, V : V : V) was developed and employed as a delivery system for kartogenin (KGN), a biocompound that can activate chondrocytes. In vitro gelation time, morphologies, swelling, weight loss, compressive modulus and cumulative release of KGN in hydrogels were studied. For biocompatibility assessments, human adipose-derived stem cells (ADSCs) were encapsulated in these hydrogels. The effects of KGN on stem cell proliferation and differentiation into nucleus pulposus-like cells were examined. The hydrogels with higher concentrations of HA showed a slightly shorter gelation time, higher water uptake, faster weight loss and faster KGN release compared to the hydrogels with lower concentrations of HA. As the KGN-conjugated hydrogel prepared with the proportions 5 : 3 : 2 displayed good mechanical properties, it was chosen as the optimal gel to promote cell proliferation and differentiation. No significant difference was seen in the expression levels of nucleus pulposus markers induced by KGN or TGF-β. Additionally, inclusion of KGN and TGF-β together did not produce a synergistic effect in inducing nucleus pulposus properties. In conclusion, we have developed a KGN-conjugated CS/HA hydrogel (5 : 3 : 2) with sustained release of KGN in hydrogel that can promote ADSC proliferation and nucleus pulposus differentiation. This kind of hydrogel may be a simple and effective candidate for the repair of degenerative NP tissue after minimally invasive surgery.
Starting Page	784
Ending Page	791
Page Count	8
File Format	HTM / HTML PDF
ISSN	20474830
Volume Number	5
Issue Number	4
Journal	Biomaterials Science
DOI	10.1039/c7bm00001d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	NP Acid Phosphorylation Intervertebral disc Stem cell Glycerol Biocompatibility Chitosan ADSC Gel
Content Type	Text
Resource Type	Article
Subject	Materials Science Biomedical Engineering

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