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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Denning, Chris Young, Lorraine E. Hammad, Moamen Rao, Wei Barrett, David A. Alexander, Morgan R. Anderson, Daniel G. Davies, Martyn C. Langer, Robert Smith, James G. W. |
| Copyright Year | 2016 |
| Abstract | Improved biomaterials are required for application in regenerative medicine, biosensing, and as medical devices. The response of cells to the chemistry of polymers cultured in media is generally regarded as being dominated by proteins adsorbed to the surface. Here we use mass spectrometry to identify proteins adsorbed from a complex mouse embryonic fibroblast (MEF) conditioned medium found to support pluripotent human embryonic stem cell (hESC) expansion on a plasma etched tissue culture polystyrene surface. A total of 71 proteins were identified, of which 14 uniquely correlated with the surface on which pluripotent stem cell expansion was achieved. We have developed a microarray combinatorial protein spotting approach to test the potential of these 14 proteins to support expansion of a hESC cell line (HUES-7) and a human induced pluripotent stem cell line (ReBl-PAT) on a novel polymer (N-(4-Hydroxyphenyl) methacrylamide). These proteins were spotted to form a primary array yielding several protein mixture ‘hits’ that enhanced cell attachment to the polymer. A second array was generated to test the function of a refined set of protein mixtures. We found that a combination of heat shock protein 90 and heat shock protein-1 encourage elevated adherence of pluripotent stem cells at a level comparable to fibronectin pre-treatment. |
| Starting Page | 1381 |
| Ending Page | 1391 |
| Page Count | 11 |
| File Format | HTM / HTML PDF |
| ISSN | 20474830 |
| Volume Number | 4 |
| Issue Number | 9 |
| Journal | Biomaterials Science |
| DOI | 10.1039/c6bm00214e |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Subscribed |
| Subject Keyword | Regenerative medicine Stem cell Tissue culture Induced pluripotent stem cell Polymer Polystyrene Protein Fibronectin Blood plasma DNA microarray Hsp90 Chemistry Heat shock protein Cell potency Biomaterial Combinatorics Mass spectrometry Fibroblast |
| Content Type | Text |
| Resource Type | Article |
| Subject | Materials Science Biomedical Engineering |
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