NDLI: Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film

Please wait, while we are loading the content...

Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film

Content Provider	Royal Society of Chemistry (RSC)
Author	Özçelik, Hayriye Lavalle, Philippe Bacharouche, Jalal Hemmerlé, Joseph Barthes, Julien François, Yannis N. Metz-Boutigue, Marie-Hélène Vrana, Nihal E. Gahoual, Rabah Francius, Grégory Schaaf, Pierre
Copyright Year	2015
Abstract	Mammalian cell culture is the starting point in many research studies focusing on biomedical applications. However, researchers have little control over the standardized cell microenvironment parameters. Here a modular ECM-mimicking surface coating for cell culture environment is designed. This substrate is a new and versatile thin film obtained by spin-coating of concentrated gelatin crosslinked by transglutaminase. It can be modified with respect to the biochemical and biophysical needs of the final cell destination, i.e. it delivers loaded multi-growth factors and serum components and allows for cell culture in a serum-free culture medium. Also, a well-known cell behavior modulator, the substrate stiffness, is controlled exogenously by addition of nanoparticles. In addition to growth factors, antimicrobial agents such as natural peptides are added to the substrate for limiting the repeated addition of antimicrobial agents to the culture medium and to prevent the increase of resistant bacterial strains in the culture environment. Finally, this substrate contains simultaneously ECM components, growth factors, stiffening elements and antimicrobial agents. It provides a favorable microenvironment and sterile conditions. It is a free-of-maintenance system, as cells will grow without addition of serum or antimicrobial cocktails. This low cost and easy-to-use substrate could emerge as a new standard for cell culture.
Starting Page	1302
Ending Page	1311
Page Count	10
File Format	HTM / HTML PDF
ISSN	20474830
Volume Number	3
Issue Number	9
Journal	Biomaterials Science
DOI	10.1039/c5bm00172b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Antimicrobial Cell culture Gelatin Extracellular matrix Transglutaminase Stiffness
Content Type	Text
Resource Type	Article
Subject	Materials Science Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Gelatin based microfluidic devices for cell culture

Culture substrates made of elastomeric micro-tripod arrays for long-term expansion of human pluripotent stem cells

In vitro strategies for mimicking dynamic cell-ECM reciprocity in 3D culture models.

The Biological and Biomechanical Role of Transglutaminase-2 in the Tumour Microenvironment

Adipose tissue-derived ECM hydrogels and their use as 3D culture scaffold

The Importance of Mechanical Forces for in vitro Endothelial Cell Biology

Matrix stiffness induces epithelial mesenchymal transition phenotypes of human epidermal keratinocytes on collagen coated two dimensional cell culture

Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers

High throughput screening to investigate the interaction of stem cells with their extracellular microenvironment

Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film

Similar Documents

Gelatin based microfluidic devices for cell culture

Culture substrates made of elastomeric micro-tripod arrays for long-term expansion of human pluripotent stem cells

In vitro strategies for mimicking dynamic cell-ECM reciprocity in 3D culture models.

The Biological and Biomechanical Role of Transglutaminase-2 in the Tumour Microenvironment

Adipose tissue-derived ECM hydrogels and their use as 3D culture scaffold

The Importance of Mechanical Forces for in vitro Endothelial Cell Biology

Matrix stiffness induces epithelial mesenchymal transition phenotypes of human epidermal keratinocytes on collagen coated two dimensional cell culture

Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers

High throughput screening to investigate the interaction of stem cells with their extracellular microenvironment

Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film