NDLI: Surfaces presenting α-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic <i>Escherichia coli</i> biofilms against pathogen colonization

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Surfaces presenting α-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic Escherichia coli biofilms against pathogen colonization

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Biomaterials Science Vol - 3 Issue - 5

Biomaterials Science Vol - 3 Issue - 4

Biomaterials Science Vol - 3 Issue - 3

Biomaterials Science Vol - 3 Issue - 2

Biomaterials Science Vol -2

Biomaterials Science Vol -1

Surfaces presenting α-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic Escherichia coli biofilms against pathogen colonization

Content Provider	Royal Society of Chemistry (RSC)
Author	Cai, Chengzhi Yu, Fei Li, Siheng Zhang, Lijuan Kumar, Amit Wang, Jun Huang, Yongkai Lopez, Analette I. Zhu, Zhiling
Copyright Year	2015
Abstract	Prevention of pathogenic colonization on medical devices over a long period of time remains a great challenge, especially in a high-nutrient environment that accelerates the production of biomass leading to biofouling of the device. Since biofouling and the subsequent pathogen colonization is eventually inevitable, a new strategy using non-pathogenic bacteria as living guards against pathogenic colonization on medical devices has attracted increasing interest. Crucial to the success of this strategy is to pre-establish a high coverage and stable biofilm of benign bacteria on the surface. Silicone elastomers are one of the most widely used materials in biomedical devices. In this work, we modified silicone surfaces to promote formation of high coverage and stable biofilms by a non-pathogenic Escherichia coli strain 83972 with type 1 fimbriae (fim+) to interfere with the colonization of an aggressive biofilm-forming, uropathogenic Enterococcus faecalis. Although it is well known that mannoside surfaces promote the initial adherence of fim+ E. coli through binding to the FimH receptor at the tip of the type 1 fimbriae, it is not clear whether the fast initial adherence could lead to a high coverage and stable protective biofilm. To explore the role of mannoside ligands, we synthesized a series of alkyl and aryl mannosides varied in the structure and immobilized them on silicone surfaces pre-coated with a poly(amidoamine) (PAMAM) dendrimer. We found that stable and densely packed benign E. coli biofilms were formed on the surfaces presenting biphenyl mannoside with the highest initial adherence of fim+ E. coli. These non-pathogenic biofilms prevented the colonization of E. faecalis for 11 days at a high concentration (108 CFU mL−1, 100 000 times above the diagnostic threshold for urinary tract infection) in the nutrient-rich Lysogeny Broth (LB) media. The result shows a correlation among the initial adherence of fim+ E. coli 83972, the coverage and long-term stability of the resulting biofilms, as well as their efficiency for preventing the pathogen colonization.
Starting Page	842
Ending Page	851
Page Count	10
File Format	HTM / HTML PDF
ISSN	20474830
Volume Number	3
Issue Number	6
Journal	Biomaterials Science
DOI	10.1039/c5bm00076a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Enterococcus faecalis Dendrimer Cell surface receptor Escherichia coli Silicone Lysogeny Broth Biomass Urinary tract infection Lysogeny broth Alkyl Biofouling Biofilm Bacteria Poly(amidoamine) Aryl Pathogen
Content Type	Text
Resource Type	Article
Subject	Materials Science Biomedical Engineering

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Surfaces Presenting α-Phenyl Mannoside Derivatives Enable Formation of Stable, High Coverage, Non-pathogenic Escherichia coli Biofilms against Pathogen Colonization.

Surfaces Presenting α-Phenyl Mannoside Derivatives Enable Formation of Stable, High Coverage, Non-pathogenic Escherichia coli Biofilms against Pathogen Colonization †

Escherichia coli and Enterococcus faecalis are able to incorporate and enhance a pre-formed Gardnerella vaginalis biofilm.

Evaluation of Antimicrobial Durability and Anti-Biofilm Effects in Urinary Catheters Against Enterococcus faecalis Clinical Isolates and Reference Strains

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Enterococcus faecalis to escherichia coli and (1994).

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Surfaces presenting α-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic Escherichia coli biofilms against pathogen colonization

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Surfaces presenting α-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic Escherichia coli biofilms against pathogen colonization